About
Synergistic effect of short- and long-read sequencing on functional meta-omics:
- assembly approaches: LR, SR and hybrid
- assembly comparison w.r.t.
- basic stat.s (length, contiguity, coverage)
- seq. similarity (incl. unique seq.s)
- genomic reconstruction (plasmids, CRISPR, rRNA genes, AMR genes)
- gene content (common/unique genes)
- GDB sample:
- metaG and metaT coverage (contigs and genes)
- functional characterization and peptide identification (w/ metaP)
Datasets
-
zymo: mock community ("EVEN") -
nwc: natural whey starter culture, a low-complexity microbiome -
rumen: metagenomic sequencing of the cow rumen -
aquifer: groundwater (complex aquifer system) -
gdb: human fecal sample- from "generous donor B": metaG (Illuminar, ONT), metaT (Illumina), metaP
-
The origins of the study
- original data prepared by Rashi (post-sequencing)
- initial analyses performed by CCL
Files
- Manuscript
- Raw data
- Processed data