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Jun 21, 2020
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Valentina Galata
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# Synergistic effect of short- and long-read sequencing on functional meta-omics
## Important info
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[
Manuscript
](
https://docs.google.com/document/d/1TmenUZGwTGU4wIljxwxprZ5L75So8hYSYL5QMzSH9_M/edit?usp=sharing
)
*NOTE: For further notes, e.g. preprocessing of raw reads, create new pages and add a link here*
## Data
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Metagenomic sequencing
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Fecal sample from "generous donor B"
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Short reads (Illumina) (SR)
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Long reads (ONT) (LR)
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Meta-omics
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metagenomics (metaG)
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metatrscriptomics (metaT)
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metaproteomics (metaP)
## Goal
SR vs. LR vs. hybri compared w.r.t.
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Genomic reconstruction
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Plasmids
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CRIPR arrays
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Functional characterization
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Peptide identification (w/ metaP)
## Analysis
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Preprocessing
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SR
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QC
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LR
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Base calling
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QC
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Assembly
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SR
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LR
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Polishing
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Hybrid
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Annotation
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Gene calling
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Plasmid prediction
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CRISPR arrays
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AMR genes
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Analysis
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Figures,tables
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Report