diff --git a/external/lab-good-practice/mycoplasma/mycoplasma.md b/external/lab-good-practice/mycoplasma/mycoplasma.md index edd717f9ef56cbf5626a278d1b272879d9bce64a..632a86a889b5dc3e4f6e4c669f6433213c3cda49 100644 --- a/external/lab-good-practice/mycoplasma/mycoplasma.md +++ b/external/lab-good-practice/mycoplasma/mycoplasma.md @@ -13,7 +13,7 @@ redirect_from: # Checking for Mycoplasma Contaminations -## What is mycoplasma and why check for mycoplasma contaminations? + Mycoplasma is a bacteria that can infect cell cultures and is one of the most common contaminants in cell culturing. @@ -46,9 +46,11 @@ Contaminated sera and other filtration products have, in the past, been a major There are two bacis testing methods for mycoplasma: 1. Direct culture in media + Direct culture is the most effective and sensitive method for detecting mycoplasma, but it is also the most difficult and time-consuming. 2. Indirect tests that measure specific characteristics of mycoplasma + There are a wide range of indirect test methods available for mycoplasma detection, including PCR-based kits, DNA fluorochome staining, autoradiography, ELISA, immunofluorescence, and specific biochemical assays. These tests are faster than direct culture and are often commercially available as kits.