From 82e89fa8a23724cfe68690001ff1b2038ba82c0b Mon Sep 17 00:00:00 2001 From: Sandy Thill <sandy.thill@uni.lu> Date: Thu, 30 Nov 2023 12:50:46 +0100 Subject: [PATCH] Update file mycoplasma.md --- external/lab-good-practice/mycoplasma/mycoplasma.md | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/external/lab-good-practice/mycoplasma/mycoplasma.md b/external/lab-good-practice/mycoplasma/mycoplasma.md index edd717f9..632a86a8 100644 --- a/external/lab-good-practice/mycoplasma/mycoplasma.md +++ b/external/lab-good-practice/mycoplasma/mycoplasma.md @@ -13,7 +13,7 @@ redirect_from: # Checking for Mycoplasma Contaminations -## What is mycoplasma and why check for mycoplasma contaminations? + Mycoplasma is a bacteria that can infect cell cultures and is one of the most common contaminants in cell culturing. @@ -46,9 +46,11 @@ Contaminated sera and other filtration products have, in the past, been a major There are two bacis testing methods for mycoplasma: 1. Direct culture in media + Direct culture is the most effective and sensitive method for detecting mycoplasma, but it is also the most difficult and time-consuming. 2. Indirect tests that measure specific characteristics of mycoplasma + There are a wide range of indirect test methods available for mycoplasma detection, including PCR-based kits, DNA fluorochome staining, autoradiography, ELISA, immunofluorescence, and specific biochemical assays. These tests are faster than direct culture and are often commercially available as kits. -- GitLab