From 5862ea7a292248f16fbbb0d4c26845b6afe49c3a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Annegr=C3=A4t=20Daujeumont?= <annegrat.daujeumont@uni.lu> Date: Thu, 5 Oct 2023 10:24:01 +0200 Subject: [PATCH] Update file ppe.md --- external/lab-hsa/ppe/ppe.md | 48 ++++++++++++++++++------------------- 1 file changed, 24 insertions(+), 24 deletions(-) diff --git a/external/lab-hsa/ppe/ppe.md b/external/lab-hsa/ppe/ppe.md index 755be100..5577302f 100644 --- a/external/lab-hsa/ppe/ppe.md +++ b/external/lab-hsa/ppe/ppe.md @@ -75,31 +75,31 @@ More informations about lab coats can be found in the dedicated [How-To card](ht ## PPE Requirements -[1. Classical laboratories activities](#1-classical-laboratories-activities) +[Classical laboratories activities](#classical-laboratories-activities) -[2. Work with strong chemicals](#2-work-with-strong-chemicals) +[Work with strong chemicals](#work-with-strong-chemicals) -[3. Work with very strong chemicals](#3-work-with-very-strong-chemicals) +[Work with very strong chemicals](#work-with-very-strong-chemicals) -[4. Autoclave unloading and other hot thermal risks](#4-autoclave-unloading-and-other-hot-thermal-risks) +[Autoclave unloading and other hot thermal risks](#autoclave-unloading-and-other-hot-thermal-risks) -[5. Automated cryostorage](#5-automated-cryostorage) +[Automated cryostorage](#automated-cryostorage) -[6. Distribution of Liquid Nitrogen in a Dewar](#6-distribution-of-liquid-nitrogen-in-a-dewar) +[Distribution of Liquid Nitrogen in a Dewar](#distribution-of-liquid-nitrogen-in-a-dewar) -[7. Usage of Liquid Nitrogen in open Dewars](#7-usage-of-liquid-nitrogen-in-open-dewars) +[Usage of Liquid Nitrogen in open Dewars](#usage-of-liquid-nitrogen-in-open-dewars) -[8. Other cold thermal risks](#8-other-cold-thermal-risks) +[Other cold thermal risks](#other-cold-thermal-risks) -[9. Spill management](#9-spill-management) +[Spill management](#spill-management) -[10. Work with non tested biological samples](#10-work-with-non-tested-biological-samples) +[Work with non tested biological samples](#work-with-non-tested-biological-samples) -[11. Work with non-toxic smelling samples](#11-work-with-non-toxic-smelling-samples) +[Work with non-toxic smelling samples](#work-with-non-toxic-smelling-samples) -[12. Working with UV light](#12-(closed)-work-with-non-tested-biological-samples) +[Working with UV light](#work-with-UV's-light) -### 1. Classical laboratories activities +### Classical laboratories activities For regular biomolecular, chemical, cell culture or microbiology work in BSL 1 or BSL 2 laboratories, the mandatory PPEs are @@ -131,7 +131,7 @@ If the gloves are not contaminated, discard them in the domestic waste bin of th <img src="img/img24.png" width="20%"> </div> -### 2. Work with toxic chemicals +### Work with toxic chemicals When handling toxic agents ex: DNA labelling reagents. @@ -162,7 +162,7 @@ Wear the glove above your lab coat. <img src="img/img24.png" height="207"> </div> -### 3. Work with very strong chemicals +### Work with very strong chemicals Dealing with strong chemicals such as strong acids, bases,... requires specific gloves providing specific protection, on top of the regular PPEs. Those gloves are not disposable gloves. @@ -181,7 +181,7 @@ Those gloves are not disposable gloves. <img src="img/img24.png" height="207"> </div> -### 4. Autoclave unloading and other hot thermal risks +### Autoclave unloading and other hot thermal risks On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 gloves** located next to the autoclaves to protect you against hot thermal hazard. Those gloves are not disposable gloves. @@ -192,7 +192,7 @@ On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 g <img src="img/img24.png" height="207"> </div> -### 5. Automated cryostorage +### Automated cryostorage On top of the mandatory PPEs for BSL1 work, it is mandatory to wear: @@ -215,7 +215,7 @@ Those gloves are not disposable gloves. <img src="img/img27.png" height="207"> </div> -### 6. Distribution of Liquid Nitrogen in a Dewar +### Distribution of Liquid Nitrogen in a Dewar On top of the mandatory PPEs for BSL1 work, it is mandatory to wear: @@ -241,7 +241,7 @@ Those gloves are not disposable gloves. <img src="img/img28.png" height="207"> </div> -### 7. Usage of Liquid Nitrogen in open Dewars +### Usage of Liquid Nitrogen in open Dewars Some experiments require to snap freeze the samples in Liquid Nitrogen or to keep samples frozen in Liquid Nitrogen during the manipulation. Having Liquid Nitrogen in an open Dewar on the bench top requires to wear on top of the regular PPEs: @@ -265,7 +265,7 @@ Those gloves are not disposable gloves. <img src="img/img27.png" height="207"> </div> -### 8. Other cold thermal risks +### Other cold thermal risks When handling samples in the -80°C freezers, in the -150°C freezer, in the cryopod or in dry-ice, it is recommended to wear, on top of the regular mandatory PPEs cotton white gloves to be worn under the regular nitrile glove. It will enable an improved dexterity compared to the regular cryo-gloves. Those cotton gloves are not disposable and should be kept in your lab coat. @@ -277,7 +277,7 @@ When handling samples in the -80°C freezers, in the -150°C freezer, in the cry <img src="img/img24.png" height="207"> </div> -### 9. Spill management +### Spill management Spill management requires extra protection that you can find in the spill kit. On top of the regular PPEs, you have to wear: @@ -313,7 +313,7 @@ Ensure protection against particles and limited protection against chemical vapo <img src="img/img24.png" height="207"> </div> -### 10. Work with non tested biological samples +### Work with non tested biological samples Collective protective equipment is always the preferred option to be protected against airborne biological hazards. Therefore, biological hazards should be manipulated under biosafety cabinets. However, if after discussion with the safety officer and/or after a risk assessment made in collaboration with the safety officer lead to the impossibility to work under a biosafety cabinet, it is mandatory to wear, on top of the regular PPEs: @@ -331,7 +331,7 @@ Ensure protection against particles and limited protection against chemical vapo <img src="img/img24.png" height="207"> </div> -### 11. Work with non-toxic smelling samples +### Work with non-toxic smelling samples To avoid unpleasant smells in the laboratories, it is recommended to work with non-toxic samples under a chemical hood or an aspiration arm. However, if after discussion with the safety officer and/or after a risk assessment made in collaboration with the safety officer lead to the impossibility to work under a chemical hood or an aspiration arm, it is recommended to wear, on top of the regular PPEs: @@ -350,7 +350,7 @@ Ensure protection against particles and limited protection against chemical vapo </div> -### 12. Work with UV's light. +### Work with UV's light. **PROTECT EYES AND SKIN FROM EXPOSURE TO UV LIGHT** -- GitLab