From 6892392a5634f2b6f7a4fcf620664d131c2da762 Mon Sep 17 00:00:00 2001
From: =?UTF-8?q?Annegr=C3=A4t=20Daujeumont?= <annegrat.daujeumont@uni.lu>
Date: Wed, 9 Aug 2023 14:40:43 +0200
Subject: [PATCH] Update file ppe.md
---
external/lab-hsa/ppe/ppe.md | 156 ++++++++++++++++++++++--------------
1 file changed, 96 insertions(+), 60 deletions(-)
diff --git a/external/lab-hsa/ppe/ppe.md b/external/lab-hsa/ppe/ppe.md
index 06f95c2a..4f5941d1 100644
--- a/external/lab-hsa/ppe/ppe.md
+++ b/external/lab-hsa/ppe/ppe.md
@@ -18,13 +18,17 @@ To protect every person present in the laboratories from any contamination or ex
Every LCSB employees, students and visitors are responsible
for wearing the approriate PPE that will vary depending on the hazards present in the room, but must always include:
-- Appropriate disposable gloves (when handling biological materials or chemicals)
+- Your **lab coat** to protect yourself as it represents an additional layer of protection for skin in case of accidental contact and splashes of hazardous substances (chemical or biological). It is also a removable barrier and it protects body’s area not completely covered by clothing.The lab coat also prevent contamination inside and outside the lab.
+
+- Appropriate **disposable gloves** (when handling biological materials or chemicals)
Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**
It is not that trivial to safely wear and remove disposable gloves without becoming contaminated, please watch the video in [DOCEBO platform](https://unisupport.docebosaas.com/learn/course/52/play/332:103/how-to-wear-and-remove-gloves;lp=3 ).
-- Specific PPEs depending on the performed activity (faceshield, thermal gloves, themal appron,...)
+ Integrity of the gloves should be checked each time you put on new gloves. The size of the gloves has to fit to ensure good protection.
+
+- **Specific PPEs** depending on the performed activity (faceshield, thermal gloves, themal appron,...)
It is highly recommended to also wear safety glasses or protective prescription glasses.
@@ -64,19 +68,24 @@ More informations about lab coats can be found in the dedicated [How-To card](ht
[11. Work with non-toxic smelling samples](#11-work-with-non-toxic-smelling-samples)
+[12. Working with UV light](#12-(closed)-work-with-non-tested-biological-samples)
+
### 1. Classical laboratories activities
For regular biomolecular, chemical, cell culture or microbiology work in BSL 1 or BSL 2 laboratories, the mandatory PPEs are
- **Lab coat**
-With sleeves fastened at the wrists, protecting the arms and torso.
+Labcoat has to be tied up to top with sleeves fastened at the wrists, protecting the arms and torso.
-Note: Disposable gloves must be discarded after each use or when they become contaminated.
- **Disposable powder free nitrile gloves**
- EN 374-1 type C (K, L, T): permeation resistance of at least 10 minutes with at least one test chemical
+Note: Disposable gloves must be discarded after each use or when they become contaminated.
+
+If the gloves are not contaminated, discard them in the domestic waste bin of the lab. Otherwise, follow the [chemical and biological waste concept](https://howto.lcsb.uni.lu/?lab:waste).
+
+ EN374-1 type B or C: Permeation resistance of at least 30/10 minutes with at least Three/one test chemical(s). We always have B, resistance to 3 products for 30min.
EN 374-5: micro-organisme risistance (bacteria, fungi, viruses)
@@ -84,13 +93,13 @@ Note: Disposable gloves must be discarded after each use or when they become con
EN 455 1-4: physical properties
-- **Safety goggles** (highly recommended)
+ #### Safety goggles (highly recommended)
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img24.png">
+<img src="img/img23.png" width="20%">
+<img src="img/img25.png" width="20%">
+<img src="img/img15.png" width="20%">
+<img src="img/img24.png" width="20%">
</div>
### 2. Work with toxic chemicals
@@ -118,10 +127,10 @@ Wear the glove above your lab coat.
- **Safety goggles** (highly recommended)
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img26.png" height="250">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img26.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 3. Work with very strong chemicals
@@ -136,11 +145,11 @@ Those gloves are not disposable gloves.
EN 388 2010X: resistance to abrasion (0-4), resistance to cut (0-5), resistance to tear (0-4), resistance to perforation (0-4)
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img10.png" height="180">
-<img src="img/img11.png" height="180">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img15.png" width="20%">
+<img src="img/img10.png" height="207">
+<img src="img/img11.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 4. Autoclave unloading and other hot thermal risks
@@ -148,10 +157,10 @@ Those gloves are not disposable gloves.
On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 gloves** located next to the autoclaves to protect you against hot thermal hazard. Those gloves are not disposable gloves.
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img1.png" height="175">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img15.png" width="20%">
+<img src="img/img1.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 5. Automated cryostorage
@@ -171,10 +180,10 @@ Those gloves are not disposable gloves.
- **Face shield**
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img2.png">
-<img src="img/img27.png" height="200">
+<img src="img/img23.png" height="207">
+<img src="img/img15.png" width="20%">
+<img src="img/img2.png" height="207">
+<img src="img/img27.png" height="207">
</div>
### 6. Distribution of Liquid Nitrogen in a Dewar
@@ -196,11 +205,11 @@ Those gloves are not disposable gloves.
- **Cryo-apron**
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img2.png">
-<img src="img/img27.png" height="200">
-<img src="img/img28.png" height="350">
+<img src="img/img23.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img2.png" height="207">
+<img src="img/img27.png" height="207">
+<img src="img/img28.png" height="207">
</div>
### 7. Usage of Liquid Nitrogen in open Dewars
@@ -220,11 +229,11 @@ Those gloves are not disposable gloves.
- **Face shield**
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img2.png">
-<img src="img/img27.png" height="200">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img2.png" height="207">
+<img src="img/img27.png" height="207">
</div>
### 8. Other cold thermal risks
@@ -232,11 +241,11 @@ Those gloves are not disposable gloves.
When handling samples in the -80°C freezers, in the -150°C freezer, in the cryopod or in dry-ice, it is recommended to wear, on top of the regular mandatory PPEs cotton white gloves to be worn under the regular nitrile glove. It will enable an improved dexterity compared to the regular cryo-gloves. Those cotton gloves are not disposable and should be kept in your lab coat.
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img6.png" width="225">
-<img src="img/img15.png" height="175">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img6.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 9. Spill management
@@ -245,7 +254,7 @@ Spill management requires extra protection that you can find in the spill kit. O
- **Chemical and cut resistant gloves**: ANSELL: AlphaTec 58-735
-To protect the skin against two type of risks: the chemicals and the glass/plasticware debris. Those gloves are to disposable.
+To protect the skin against two type of risks: the chemicals and the glass/plasticware debris. Those gloves are disposable.
ANSI A5 ABR: heavy resistance to abrasion (10,000 - 19,999 cycles)
@@ -266,13 +275,13 @@ Ensure protection against particles and limited protection against chemical vapo
EN 149:2001 + A1:2009: filtrationof at least 94% of the particules.
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img12.png" height="230">
-<img src="img/img13.png" height="230">
-<img src="img/img19.png" height="230">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img12.png" height="207">
+<img src="img/img13.png" height="207">
+<img src="img/img19.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 10. Work with non tested biological samples
@@ -286,11 +295,11 @@ Ensure protection against particles and limited protection against chemical vapo
EN 149:2001 + A1:2009: filtrationof at least 94% of the particules.
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img19.png" height="230">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img19.png" height="207">
+<img src="img/img24.png" height="207">
</div>
### 11. Work with non-toxic smelling samples
@@ -304,9 +313,36 @@ Ensure protection against particles and limited protection against chemical vapo
EN 149:2001 + A1:2009: filtrationof at least 94% of the particules.
<div align="center">
-<img src="img/img23.png" height="350">
-<img src="img/img25.png" height="350">
-<img src="img/img15.png" height="175">
-<img src="img/img19.png" height="230">
-<img src="img/img24.png">
+<img src="img/img23.png" height="207">
+<img src="img/img25.png" height="207">
+<img src="img/img15.png" height="207">
+<img src="img/img19.png" height="207">
+<img src="img/img24.png" height="207">
</div>
+
+
+### 12. Work with UV's light.
+
+**PROTECT EYES AND SKIN FROM EXPOSURE TO UV LIGHT**
+
+- **Labcoat**
+
+Body needs to be fully covered with long trouser, closed shoes and labcoat. Labcoat has to be tied up to top with sleeves fastened at the wrists, protecting the arms and torso. Lab workers must be particularly vigilant to prevent gaps in protective clothing that commonly occur around the neck and wrist areas.
+
+- **Disposable powder free nitrile gloves**
+
+Hands must be protected with long purple nitrile gloves to ensure protection of the wrist.
+
+ EN 374-1 type C (K, L, T): permeation resistance of at least 10 minutes with at least one test chemical
+
+ EN 374-5: micro-organisme risistance (bacteria, fungi, viruses)
+
+ EN 420: general requirements (identification, size, norms, composition,...)
+
+ EN 455 1-4: physical properties
+
+- **Faceshield**
+
+As soon as UV light is switched on, face shield is mandatory (if the protective glass of the equipment can not be closed completely). All the eye protection available in the lab will not fit for this purpose. Attention need to be paid to the specification of the PPE, in accordance with the safety officer.
+
+EN170 2002 (2 or 3) (protection filter against UV)
\ No newline at end of file
--
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