diff --git a/.gitattributes b/.gitattributes
index c139e44b4dc5f5fdd3e6d7c6980367582dd7a808..b2f4a6e23972bf6dd39042b6acbdfe6270ab2e4e 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1,3 +1,4 @@
*.png filter=lfs diff=lfs merge=lfs -text
*.jpg filter=lfs diff=lfs merge=lfs -text
*.jpeg filter=lfs diff=lfs merge=lfs -text
+*.mp4 filter=lfs diff=lfs merge=lfs -text
diff --git a/Gemfile b/Gemfile
index fa0d4eb5cfdcc24a7dc79b4eda810acc2c6ab05a..7d4e5e8f1cb5f12c0237c1a22df7c48056ab0f20 100644
--- a/Gemfile
+++ b/Gemfile
@@ -45,4 +45,4 @@ gem "tzinfo-data", platforms: [:mingw, :mswin, :x64_mingw, :jruby]
# Performance-booster for watching directories on Windows
gem "wdm", "~> 0.1.1" if Gem.win_platform?
-gem 'jekyll-spaceship', "0.6.0"
+gem 'jekyll-spaceship', "0.9.9"
diff --git a/cards.md b/cards.md
index 320ddda8fa8da2e328cbdbc912bc8ed1bc41df22..0689f8e2ae1d06021329b9b405badd78424595af 100644
--- a/cards.md
+++ b/cards.md
@@ -79,6 +79,20 @@ order: -1
<li><a href="external/integrity/encryption/file">Encrypting Files and Folders</a></li>
<li><a href="external/integrity/naming">Naming files</a></li>
<li><a href="external/integrity/organization">Organization</a></li>
+ <li><a href="external/integrity/spreadsheets">Working with spreadsheets</a></li>
+
+ </ul>
+ </div>
+ <div class="index-box noborderbox" id="lab">
+ <h3>Lab</h3>
+ <ul>
+ <li><a href="external/lab/book-lab-equipment">How to book a Lab Equipment in Quarks</a></li>
+ <li><a href="external/lab/dishwasher-utilization-and-maintenance">Dishwasher utilization and maintenance</a></li>
+ <li><a href="external/lab/handwashing">Handwashing</a></li>
+ <li><a href="external/lab/maintenance_of_cold_traps">Maintenance of cold traps </a></li>
+ <li><a href="external/lab/personal-alert-safety-system">Personal alert safety system (PASS)</a></li>
+ <li><a href="external/lab/utilization-of-balances">Utilization of balances</a></li>
+ <li><a href="external/lab/utilization-of-pH-meter">Utilization of pH meter</a></li>
</ul>
</div>
diff --git a/external/integrity/encryption/cloud/cloud.md b/external/integrity/encryption/cloud/cloud.md
index a08f8e70610c8977167f0369352369c26ef54ea5..4b9cbad1ffcc62944c355ba6d239c2737905e936 100644
--- a/external/integrity/encryption/cloud/cloud.md
+++ b/external/integrity/encryption/cloud/cloud.md
@@ -9,6 +9,52 @@ redirect_from:
# Data upload to cloud
-Data encryption and hash-check for uploading to UL-LCSB onedrive or owncloud
+Data encryption and hash-check for uploading to UL-LCSB onedrive or owncloud from Windows.
-[Windows](https://git-r3lab.uni.lu/transmed/support/data-upload-to-cloud/-/wikis/Upload-data-from-Windows)
+| Requirements | Restrictions |
+| ------ | ------ |
+| Windows 8 or newer (recommended Windows 10) | Recommended for data size less than 100 GB |
+| RAM 2GB (recommended 4GB) | If transfer through Aspera is not possible |
+| Enough free disc space (data will be duplicated for encryption) | Larger data will take more time to encrypt |
+| Administrative rights to install software | |
+| Web browser (Firefox, Edge or Chrome) | |
+
+## Install `7zip` for encryption
+
+Download and install `7zip` from [here](https://www.7-zip.org/)
+
+## Install tool for MD5 checksum calculation
+
+1. Search Microsoft Store for `Simple Checksum Calculator` or go to this [link](https://www.microsoft.com/en-us/p/simple-checksum-calculator/9nblggh5l6t9?activetab=pivot:overviewtab)
+2. Install `Simple Checksum Calculator`
+
+
+## Encrypt your data with `7zip`
+
+1. Right click on the folder to encrypt > 7zip > Add to archive
+2. Select following values:
+ - Archive format: `zip`
+ - Compression: `store`
+ - Check `Show Password` box
+ - Encryption: `AES256`
+3. Enter a password and make a copy of the encryption password :key:
+4. Click save to save the encrypted zip file
+
+
+
+## Calculate the MD5 checksum with `Simple Checksum Calculator`
+
+1. Open `Simple Checksum Calculator` from the start-menu
+2. Select `File` tab from the top menu
+3. Click on the file icon box in the left panel and load the encrypted zip file
+4. Make a copy of the MD5 checksum key :label:
+
+
+
+## Data upload
+
+1. Open the `OneDrive` or `ownCloud` URL link that you have received from UL-LCSB contact
+2. Upload the encrypted zip file
+3. After the upload completion, open [UNILU privatebin](https://hpc.uni.lu/privatebin/)
+4. Paste the **encryption password :key:** and **MD5 checksum :label:** in the Editor box and press send
+5. Copy the privatebin URL and send it your UL-LCSB contact through email
\ No newline at end of file
diff --git a/external/integrity/encryption/cloud/media/7zip-encryption-windows.mp4 b/external/integrity/encryption/cloud/media/7zip-encryption-windows.mp4
new file mode 100644
index 0000000000000000000000000000000000000000..b538c7be740713e704a26912100f8bacd9678c02
--- /dev/null
+++ b/external/integrity/encryption/cloud/media/7zip-encryption-windows.mp4
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:2c0bf159b00835787727434c57dc0dcc3226806d187dbea6b9aa5ef52f898cbb
+size 3898438
diff --git a/external/integrity/encryption/cloud/media/Get_MD5_checksum_windows.mp4 b/external/integrity/encryption/cloud/media/Get_MD5_checksum_windows.mp4
new file mode 100644
index 0000000000000000000000000000000000000000..82469e166fb21b218fe5e6b8c53d9a8d489f9863
--- /dev/null
+++ b/external/integrity/encryption/cloud/media/Get_MD5_checksum_windows.mp4
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:80b3f6ac59d15cd19f9a6bae01d9cfd7445f5df804fb131c679e563497900199
+size 3459630
diff --git a/external/integrity/spreadsheets/img/excel_analyses-sheet.jpeg b/external/integrity/spreadsheets/img/excel_analyses-sheet.jpeg
new file mode 100644
index 0000000000000000000000000000000000000000..1e24b5575fa05d9e6e7aabb3cb2eaa3bc3251301
--- /dev/null
+++ b/external/integrity/spreadsheets/img/excel_analyses-sheet.jpeg
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:b17184a42b86f91bd4067c4bd00f9fdd717ed38ba60df1eca0c3c4f421c0c4a1
+size 122967
diff --git a/external/integrity/spreadsheets/img/excel_data-sheet.png b/external/integrity/spreadsheets/img/excel_data-sheet.png
new file mode 100644
index 0000000000000000000000000000000000000000..63a824bcefb1c303f813ec5367400664496ee2aa
--- /dev/null
+++ b/external/integrity/spreadsheets/img/excel_data-sheet.png
@@ -0,0 +1,3 @@
+version https://git-lfs.github.com/spec/v1
+oid sha256:dace0d7847c2fbe29a49869180b58697ceeaa8633d7f1d570fe4e8910542ba9f
+size 26789
diff --git a/external/integrity/spreadsheets/spreadsheets.md b/external/integrity/spreadsheets/spreadsheets.md
new file mode 100644
index 0000000000000000000000000000000000000000..c5146a650fdabec45bf6691cd9169ca7be763bf8
--- /dev/null
+++ b/external/integrity/spreadsheets/spreadsheets.md
@@ -0,0 +1,79 @@
+---
+layout: page
+permalink: /external/integrity/spreadsheets/
+shortcut: integrity:spreadsheets
+redirect_from:
+ - /cards/integrity:spreadsheets
+ - /external/cards/integrity:spreadsheets
+---
+
+# Working with spreadsheets
+
+Spreadsheets are widely used tools for tabular data manipulation facilitating data input and allowing simple formatting, validation and visualization.
+
+This card describes tabular data format, common mistakes made in spreadsheets and how to use your spreadsheet application effectively while increasing re-usability, quality and accuracy of your data.
+
+## What is a table (tabular data format)?
+
+Data in tabular format follow 3 key conditions:
+
+ 1. table contains a one-line header containing unique machine readable column names
+ 2. table has rows representing individual observations
+ 3. table has columns representing attributes/features of the observations and contain values of one data type
+
+| Table | Not a table |
+|:-----------------------------------|---------------------------|
+|<img src="./img/excel_data-sheet.png" height=200> | <img src="./img/excel_analyses-sheet.jpeg" height=200>|
+
+## Tips and Tricks
+
+### Keep the original
+
+Changes in spreadsheets are not tracked. Any update or change should produce a new file labeled by version with changes described in change log.
+
+### Export data after collection
+
+For reproducibility purposes, the collected data should be always exported from proprietary (.xlsx, .xls, ...) format into non-proprietary format (.csv, .tsv, etc.) with minimal metadata in README file.
+
+### Cell
+
+- Use field validation - validation rules on columns ensure you have data checked automatically already on input.
+- Avoid non-exportable proprietary content - visual formatting (cell coloring / outlining), embedded comments and charts, merged cells, ...
+
+### Table
+
+- Keep header column names machine readable. You can follow the same best practices as for file naming (see our [Card on file naming](../naming/file_naming.md)).
+- Keep values in columns atomic.
+- Use primary keys - values in one particular column should be unique for the whole table. This will allow you to create unique references pointing to one and only one observation/record.
+- Do not insert empty rows or columns which would split the table in two.
+- Keep data in long format (sometimes referred to as narrow, gathered or melted format). All columns should be meaningful for all observations. If a new observation requires a new column to be created or if the observation's data ends up in just one cell instead of the whole row, your table is most probably **not** in long format.
+
+#### MS Excel Table tool
+
+MS Excel feature called **Table** (found in Insert->Table) allows to create real table object instead of just cell range. Its main advantages are:
+
+- Table formatting and column validation is expanded automatically with a new observation/record.
+- Each table object can be referenced by its name - no more (named) cell ranges and hard-to-read formulas.
+- Automatically adds filter buttons and subtotals.
+
+### Sheet
+
+- Keep one table per sheet or workbook.
+- Start your table in the first column (and preferably on the first row).
+- Do not insert any values next or below your table - add the content to a new column, new table, analyses sheet or file with metadata.
+- Keep metadata about the table in separate sheet in tabular format or separate file. If you must, keep metadata **above** the table itself.
+
+### Analyses
+
+- Keep data separate from the analyses - create a link to the data from sheets or workbooks containing the analyses.
+- Use pivot tables - if your data is in long format (it should be), it is very easy to create dynamic summary tables.
+- Use pivot charts - you can produce your desired auto-refreshing charts while having data still in long format.
+- Script more advanced analyses and data manipulation using standard tools for data processing (R, Python, Bash, ...).
+
+## Further reading
+
+Data Curation Network - [Microsoft Excel Data Curation Primer](https://github.com/DataCurationNetwork/data-primers/blob/master/Excel%20Data%20Curation%20Primer/Excel%20Data%20Curation%20Primer.md)
+
+Data Carpentry - [Spreadsheet ecology lesson](https://datacarpentry.org/spreadsheet-ecology-lesson/)
+
+Wikipedia - [Wide and narrow data](https://en.wikipedia.org/wiki/Wide_and_narrow_data)
diff --git a/external/lab/book-lab-equipment/book-lab-equipment.md b/external/lab/book-lab-equipment/book-lab-equipment.md
new file mode 100644
index 0000000000000000000000000000000000000000..5c2cb8a4b9c4edb758f9f6facb2b58a078a8ec49
--- /dev/null
+++ b/external/lab/book-lab-equipment/book-lab-equipment.md
@@ -0,0 +1,153 @@
+---
+layout: page
+permalink: /external/lab/book-lab-equipment/
+shortcut: lab:book-lab-equipment
+redirect_from:
+ - /cards/lab:book-lab-equipment
+ - /external/cards/lab:book-lab-equipment
+---
+
+# How to book a Lab Equipment in Quarks
+
+To help the lab users to plan their work and to ensure that a lab equipment will be free to use, the Quarks software provides a booking tool. This booking tool is also used by the support team to indicate the maintenance periods, or if an equipment is not available due to a failure or a breakdown.
+
+ - [1. How to look for an instrument](#1-how-to-look-for-an-instrument)
+ - [2. How to know if an instrument is bookable](#2-how-to-know-if-an-instrument-is-bookable)
+ - [3. How to add an equipment to your Favorites list](#3-how-to-add-an-equipment-to-your-favorites-list)
+ - [4. How to book an instrument](#4-how-to-book-an-instrument)
+ - [5. How to edit or cancel a booking](#5-how-to-edit-or-cancel-a-booking)
+ - [6. How to book a lab (location)](#6-how-to-book-a-lab-(location))
+ - [7. How to access the booking history](#7-how-to-access-the-booking-history)
+ - [8. How to request booking configuration change or report an issue with Quarks?](#8-how-to-request-booking-configuration-change-or-report-an-issue-with-Quarks?)
+
+## 1. How to look for an instrument
+
+In your Quarks account, go on the _Equipment_ platform. In the _Equipment_ section, you have a search tool to help you find an equipment among all the LCSB equipment. You can search by internal reference number (e.g. LCSB01685), model, manufacturer or serial number in the “quick search†field.
+If you want to add more search criteria, you can do so in the drop down list “Add a filterâ€.
+
+
+
+## 2. How to know if an instrument is bookable
+
+You can easily know if the instrument you are looking for is bookable or not thanks to the calendar symbol in the “Actions†column.
+
+
+
+You can also see if the instrument is bookable or not on the side panel that will appear by clicking on the instrument itself, in the reservation section. Instrument that might be used for long experiment, possibly overnight, have the “24h bookable†option.
+
+
+
+A bookable instrument will have a calendar icon available. Typically, only specific equipment or equipment that is usually used for long experiments is bookable.
+
+## 3. How to add an equipment to your Favorites list
+
+In the “Actions†tools, you have the option to add an instrument in your favorite list by clicking on the heart.
+
+
+
+To have a direct access to your favorite equipment, go in “Equipment†– “My favoritesâ€.
+
+
+
+For your favorite equipment, you have the option to receive notifications by email. You will receive a notification if someone has edited or cancelled a booking just before yours, like in the example below. You can decide to activate this function for equipment heavily booked.
+
+
+
+To receive the notifications, click on the edit icon and select Yes for the Notifications.
+
+
+
+
+
+You can directly see for which instrument you have the notifications activated by looking in the Notifications column.
+
+
+
+## 4. How to book an instrument
+
+You have three ways to create a new booking.
+1. You can book your instrument by clicking on its name, you will have in the right window a reservation section.
+
+ 
+
+ 
+
+ You can select the date and time of your booking and also create a recurrent booking in the Periodicity section.
+
+2. You can also directly access the reservation window by clicking on the “Calendar†icon.
+ 
+
+3. The other way of making a new reservation is in the “Reservation†– “Agenda†section.
+
+ 
+
+ In the “Type†field, select Equipment. You can filter the equipment either by your Favorites and/or by using the different Conditional criteria.
+
+ 
+
+ In “Bookable itemâ€, you can write the name of the equipment and you will have drop down list of corresponding equipment.
+
+ 
+
+ After you select the date of the booking (steps 1 to 3), click on the raw of your equipment. The “New reservation†side panel will open on the right. You can now create your booking and set up a Periodicity if needed.
+
+ **Step 1**
+
+ 
+
+ **Step 2**
+
+ 
+
+ **Step 3**
+
+ 
+
+ **Step 4**
+
+ 
+ 
+ 
+
+ By Clicking on the name of the equipment, you can have a weekly overview of the bookings for this equipment. You can also create a booking from here, by clicking on the calendar.
+
+ 
+
+## 5. How to edit or cancel a booking
+
+You can edit a booking by clicking on the time period. The right window will be displayed.
+
+
+
+
+
+Click on “Edit†and you will be able to change the date and time. If you click on “Cancelâ€, the entire booking will be cancelled.
+
+
+
+You can also edit or cancel your booking in the “Reservations†– “ Reservation†section. Click on the equipment name and the right window with your booking information will be displayed.
+
+
+
+
+
+## 6. How to access the booking a lab (location)
+
+In some cases the equipment reservation is organized via lab booking. If you want to reserve a lab please select under Reservations “Locations†instead of “Equipment†as Type. The further way of proceeding is similar to described above for Equipment reservations.
+
+
+
+
+## 7. How to access the booking history
+
+You can have an overview of all the bookings in the “Reservations†– “Reservations†section. You can filter them by date, type (Location or Equipment), name (Bookable item) and person who have made the booking (Reserved by).
+
+
+
+
+## 8. How to request booking configuration change or report an issue with Quarks?
+
+If you wish to make one equipment bookable or make it 24 hours bookable [please send a ticket](https://service.uni.lu/sp?id=sc_cat_item_lsmc&sys_id=1dac368d54be72000a5374545c1c6a62&business_service_id=014ebe3ddb6a14148bcbf9b41d96195e&action_id=ec38cb7ddbaa14148bcbf9b41d9619a3).
+
+For all issues related to Quarks [please send a ticket](https://service.uni.lu/sp?id=bs_dep_catalog_entry&sys_id=0840cf71dbaa14148bcbf9b41d9619cb).
+
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diff --git a/external/lab/dishwasher-utilization-and-maintenance/dishwasher-utilization-and-maintenance.md b/external/lab/dishwasher-utilization-and-maintenance/dishwasher-utilization-and-maintenance.md
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+---
+layout: page
+permalink: /external/lab/dishwasher-utilization-and-maintenance/
+shortcut: lab:dishwasher-utilization-and-maintenance
+redirect_from:
+ - /cards/lab:dishwasher-utilization-and-maintenance
+ - /external/cards/lab:dishwasher-utilization-and-maintenance
+---
+
+# Dishwasher utilization and maintenance
+
+Utilization of the Miele dishwashers on the 5th floor of BT1 and on the 1st floor of BT2.
+
+## 1. Description
+
+<div align="center">
+<img src="img/dishwasher_img_1.jpg" height="400">
+</div>
+
+1. Control panel
+2. Serial interface
+3. Water intake
+4. Sensor access for validation
+5. Salt container port
+6. Filter combination
+7. Service panel
+8. Containers for DOS 2 and DOS 4 dispensing systems (optional)
+9. DOS drawer
+10. Containers for neutralize (red) and liquid detergent (blue)
+11. Drying unit
+12. Reset button
+
+<div align="center">
+<img src="img/dishwasher_img_2.jpg" height="200">
+</div>
+1. On/Off button
+2. Door release
+3. Display
+4. Optical interface
+5. Start button
+6. Clear button
+7. Selection button: up
+8. OK button
+9. Selection button: down
+
+## 2. Utilization
+
+### **1. Loading the dishwasher**
+
+1. Ensure that no acid or solvent residues, especially hydrochloric acid or chlorides, get inside the machine
+2. Empty all glassware before loading into the machine
+3. Remove all agar residues
+4. Remove all labels, sealing was residues and handwritten text on the bottles
+5. Small parts should be secured in basket inserts
+6. Load your items on the tray
+7. Close the door
+
+### **2. Selecting a program and running the dishwasher**
+
+1. Click on <img src="img/dishwasher_img_3.jpg"> to switch on the instrument
+2. Select the “Chemistry Bio†program using the up and down arrows
+
+ <div align="center">
+ <img src="img/img/../dishwasher_img_4.jpg">
+ </div>
+
+3. Click on <img src="img/dishwasher_img_5.jpg"> to start the program
+
+### **3. Cancel a program during run**
+
+1. To cancel the program, click on <img src="img/dishwasher_img_6.jpg">
+2. The program is then interrupted
+3. At water temperatures below 40°C, the following message appears on the display: “Program cancel (OK) or Continue (Clear)?â€
+4. At water temperatures above 40°C, the following message appears on the display: “Cancel program (OK)â€
+5. Confirm the program cancellation with <img src="img/dishwasher_img_7.jpg">
+6. The following message appears on the display: “Program cancelled. Water drainageâ€
+7. After the water has drained away, the program list returns to the display
+
+### **4. Unloading**
+
+1. When the program is finished, the message “End of program†is displayed on the screen
+2. Click on <img src="img/dishwasher_img_8.jpg"> to open the door
+3. Unload the tray and store the items in the cupboards
+
+## 3. Maintenance
+
+### **1. Reactivation**
+1. This section is available in the dishwasher room
+2. If the display shows “Reactivationâ€, salt needs to be added to the dishwasher **directly when the message appears! Do not run a cycle when reactivation is needed!**
+3. Material needed: salt and container
+ <div align="center">
+ <img src="img/dishwasher_img_9.jpg" height="200">
+ </div>
+
+4. To fill the container, unscrew and remove the filter insert from the container
+ <div align="center">
+ <img src="img/dishwasher_img_10.jpg" height="200">
+ </div>
+
+5. Completely fill the salt container with reactivation salt (Miele, 7785780;LIMS ID: 3278) and replace the filter insert. The reactivation will only be effective if the container is completely full.
+
+ <div align="center">
+ <img src="img/dishwasher_img_11.jpg" height="300">
+ </div>
+
+6. Remove any mobile racks from the dishwasher cabinet
+ <div align="center">
+ <img src="img/dishwasher_img_12.jpg" height="250">
+ </div>
+
+7. Unscrew the plastic cap located at the top right-hand of the cabinet. A small amount of residual water will be in the cap. Take care, as it might be hot from the previous program.
+ <div align="center">
+ <img src="img/dishwasher_img_13.jpg" height="300">
+ </div>
+
+8. Screw the salt container firmly onto the socket
+ <div align="center">
+ <img src="img/dishwasher_img_14.jpg" height="300">
+ </div>
+
+9. Close the door
+10. Select and start the “Reactivation†program
+11. The system will automatically perform the reactivation
+12. When the cycle is finished, open the door
+13. Carefully unscrew the salt container in order to allow any water pressure to subside. Do not use force, if the container cannot be removed manually, contact the lab maintenance team
+14. The salt container must be emptied in the sink
+15. Screw the softener lid back on
+16. Insert the mobile unit
+17. Wash and rinse the salt container and filter cap with clear water
+
+**Notes**:
+
+* BT1: the salt is stored in the lower cupboard next to the right dishwasher
+
+* BT2: some boxes are available in the cupboard above the sink and the main stocks are in the -2 stock room
+
+### **2. Routine checks**
+
+#### 1. The filters in the base of the wash cabinet
+
+1. The filters of the cabinets need to be checked and cleaned regularly
+2. To clean the coarse filter, press the two lugs together, remove and clean the coarse filter
+
+ <div align="center">
+ <img src="img/dishwasher_img_15.jpg" height="300">
+ </div>
+
+3. To clean the flat and micro-fine filters,
+4. Remove the fine filter which sits inside the micro-fine filter
+
+ <div align="center">
+ <img src="img/dishwasher_img_16.jpg" height="300">
+ </div>
+
+5. To unscrew the micro-fine filter, turn twice counter clockwise
+
+ <div align="center">
+ <img src="img/dishwasher_img_17.jpg" height="300">
+ </div>
+
+6. Then, pull out the micro-fine filter together with the flat filter
+7. Clean the filter
+8. Replace the filters by performing the above steps in the reverse order
+9. Ensure that the filters sit flat in the base of the wash cabinet
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diff --git a/external/lab/handwashing/handwashing.md b/external/lab/handwashing/handwashing.md
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+---
+layout: page
+permalink: /external/lab/handwashing/
+shortcut: lab:handwashing
+redirect_from:
+ - /cards/lab:handwashing
+ - /external/cards/lab:handwashing
+---
+# Handwashing
+
+The procedure aims to describe the good practices in handwashing before and after laboratory manipulations.
+
+## Description
+
+1. Handwashing is performed before doing any experiment in the lab as well as before exiting the laboratory.
+
+2. A good handwashing is essential to avoid contamination and must last at least 40 seconds.
+
+3. Consider the sink, including the faucet, as contaminated and avoid direct contact with them.
+
+4. Wet your hands with water.
+
+5. Dispense a dose of soap in the palm of your hand.
+
+6. Rub your hands palm to palm.
+
+7. Rub your right palm over left dorsum with interlaced fingers and vice versa.
+
+8. Rub palm to palm with fingers interlaced.
+
+9. Rub the back of your fingers to the opposing palms with fingers interlocked.
+
+10. Perform a rotational rubbing of the left thumb clasped in the right palm and vice versa.
+
+11. Perform a rotational rubbing backwards and forwards with clasped fingers of the right hand in the left palm and vice versa.
+
+12. Rinse your hands with water.
+
+13. Dry your hands thoroughly with a single use towel.
+
+14. Use the towel to turn off the faucet
+
+<div align="center">
+<img src="img/handwashing_img_1.png">
+</div>
+
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diff --git a/external/lab/maintenance_of_cold_traps/maintenance_of_cold_traps.md b/external/lab/maintenance_of_cold_traps/maintenance_of_cold_traps.md
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+---
+layout: page
+permalink: /external/lab/maintenance_of_cold_traps/
+shortcut: lab:maintenance_of_cold_traps
+redirect_from:
+ - /cards/lab:maintenance_of_cold_traps
+ - /external/cards/lab:maintenance_of_cold_traps
+---
+# Maintenance of cold traps
+
+## Labconco Centrivap concentrator and cold trap
+
+The procedure describes the steps for routine maintenance of the Labconco Centrivap concentrators and cold traps.
+This maintenance is important to preserve the integrity of the glass bottles inside the cold trap.
+
+**The Instrument care officer** is responsible for cleaning the condenser once a year, changing the acid filter when necessary and filling the cold trap maintenance record sheet.
+
+**The Users** are responsible for the routine maintenance of the instrument and filling the “cold trap maintenance record sheetâ€.
+
+## Description
+
+To ensure proper functioning of the centrivap concentrators and associated cold trap, a routine maintenance is necessary:
+
+<div align=center>
+
+**_Personal Protective Equipment_**:
+Lab coat
+Nitrile gloves
+
+</div>
+
+1. This routine maintenance is performed by the users of the instrument.
+
+2. If a glass bottle is used inside the cold trap, add 99% ethanol to the stainless steel trap until the glass bottle is at least two-thirds immersed.
+
+3. At the end of each week, the cold trap should be switched off and the condensed solvent in the glass bottle should be discarded in the corresponding waste container.
+
+4. If there is any ice in the stainless steel trap (outside the glass bottle), let it melt, drain the ethanol and replace it with fresh ethanol.
+To drain, use the faucet on the left side of the cold trap.
+
+<div align="center">
+<img src="img/cold-trap_img_1.png" height="250">
+</div>
+
+5. Fill the “cold trap maintenance record sheet “ (Appendix 01)
+
+6. Restart the cold trap at the beginning of the following week
+
+**Once a year, the refrigeration system condenser of the Cold trap should be cleaned.**
+
+<div align=center>
+
+**_Personal Protective Equipment_**:
+Lab coat
+Nitrile gloves
+Safety goggles
+
+</div>
+
+1. This step is performed by a maintenance officer
+
+2. Switch off the instrument
+
+3. Use a vacuum cleaner with brush attachment
+
+4. Clean the condenser (on the left side of the cold trap) to ensure proper airflow for peak performance
+
+<div align="center">
+<img src="img/cold-trap_img_2.png" height="250">
+</div>
+
+5. Fill the “cold trap maintenance record sheet “ (Appendix 01)
+When necessary, the acid cartridge (LIMS ID: 10608) should be exchanged
+
+* When the cartridge looks wet (see arrow on the following picture), it should be exchanged for a new one.
+
+<div align="center">
+<img src="img/cold-trap_img_3.png" height="250">
+</div>
+
+* This is done by a maintenance officer
+
+* Switch off the instrument
+
+* Unscrew the black cap out of the transparent container
+
+* Remove the cartridge and replace it with the new one
+
+* Close back the container
+
+* Place the old cartridge in the chemical hood for evaporation in a retention container
+
+* 24 hours later, trash it in a blue bin UN3291
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diff --git a/external/lab/personal-alert-safety-system/personal-alert-safety-system.md b/external/lab/personal-alert-safety-system/personal-alert-safety-system.md
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+---
+layout: page
+permalink: /external/lab/personal-alert-safety-system/
+shortcut: lab:personal-alert-safety-system
+redirect_from:
+ - /cards/lab:personal-alert-safety-system
+ - /external/cards/lab:personal-alert-safety-system
+---
+
+# Personal alert safety system (PASS)
+
+Utilization of the PASS “Loner M6†(BlackLineSafety) available in BT1 and BT2.
+PASS is a device used by a person working alone in a potentially dangerous environment.
+The device allows calling for rescue in case of problem.
+The call can be triggered voluntarily or automatically, in the event of a fall for example.
+
+The lone worker should pick up and register the utilization of the PASS in the logbook.
+After use, they should ensure the device is correctly plugged to charge the battery.
+
+## 1. Overview
+
+1. The phones detect if the user is:
+
+ a. falling
+ b. immobile for more than 5 minutes
+
+2. Therefore, the PASS automatically sets off a pre-alarm, which warns the user.
+If this pre-alarm is not reset, the PASS calls the emergency number 5555.
+
+3. In case of emergency, the PASS can also be used to call manually 5555.
+
+4. It is recommended when:
+
+ a. working in isolated rooms,
+ b. performing dangerous activities
+ c. working alone during the evenings or weekends
+
+5. It is forbidden to perform dangerous experiments during the night and weekends (including work with Piranha solution, highly corrosive or explosive compounds).
+
+## 2. Handling the device
+
+### 1. Description of the System
+
+<img src="img/pass_img-1.jpg">
+
+### 2. Quick user guide
+
+<img src="img/pass_img-2.jpg">
+
+### 3. How to obtain the PASS
+
+1. The PASS are stored by the guard stations in both BT1 and BT2.
+
+ <div align="center">
+
+ <img src="img/pass_img-3.jpg" height="300">
+
+ <img src="img/pass_img/../pass_img-4.jpg" height="300">
+
+ <img src="img/pass_img-5.jpg" height="300">
+
+ </div>
+
+2. To obtain the PASS, place your BT2 badge in front of the lock until the light is green.
+
+3. Fill in the logbook
+
+### 4. Switch on
+
+1. Press on the power button
+
+2. A single alarm will sound, the alarm light array will flash, and the device will vibrate once.
+The power button and SureSafe indicator light (green) will continue to flash while establishing a network connection.
+
+3. Wait for the device to establish a network connection, which can take up to one minute.
+
+4. Once connected, the SureSafe indicator light will remain continuously on
+
+### 5. Fastening the emergency phone to the clothing
+
+5. The PASS can be fastened to the clothes using the clip located in the back of the unit
+
+6. Hold the PASS in a vertical position while fastening it
+
+### 6. Switch off
+
+7. Press and hold the power button for approximately 3 seconds (until the device stops beeping).
+
+8. The alarm will sound three long beeps while the power button is pressed the device vibrates for three long pulses.
+
+The alarm, vibration motor and power button and SureSafe indicator light will turn off after the shutdown is complete.
+
+### 7. Return the PASS
+
+9. When you are done with the PASS, place it back on the charger, a red light will flash to indicate that the battery is charging.
+
+10. Fill the logbook and close the box by placing your BT2 badge in front of the lock.
+
+## 3. Types of alerts
+
+### 1. Emergency alert
+
+1. Pull the emergency latch. The device will instantly send an emergency message to the 5555.
+
+2. The device will trigger the “high alarm†pattern to notify others nearby of the event.
+
+3. To silence the alert, return the emergency latch to the closed position.
+
+4. Press and hold the acknowledge button until the alarm pattern is silenced (approximately 3 seconds).
+The device will return to regular operation.
+
+5. <img src="img/pass_img-6.jpg"> silencing the alert will not cancel it: the 5555 will still intervene.
+
+### 2. Fall impact detected alert
+
+The device is constantly monitoring your motion or falls. If the device detects a fall impact, it will trigger the “pending alarm†mode.
+
+If you do not require assistance, you have 30 seconds to cancel the alert by pressing on the acknowledge button.
+
+1. Cancelling a pending fall detected alert
+
+ a. The device will start a pending alarm
+
+ b. Press the acknowledge button until the alarm pattern is silenced (approximately 1 second).
+ The device will return to regular operation
+
+2. Silencing a fall detected alert
+
+ a. If you have not cancelled the alert within 30 seconds, the fall detected alert is communicated to the 5555
+
+ b. The device will go to “high alarmâ€
+
+ c. Press and hold the acknowledgement button until the alarm pattern is silenced (approximately 3 seconds).
+ The device will return to regular operation
+
+ d. <img src="img/pass_img-6.jpg"> Silencing an alarm will not cancel the call, 5555 will still intervene.
+
+### 3. No-motion alert
+
+The device is constantly monitoring your motion to detect a possible person down incident.
+If the device has not detected motion within 5 minutes, it will prompt you to acknowledge your safety, entering the alarm mode and triggering the low alarm pattern.
+If you do not require assistance, you have 30 seconds to cancel the alert by pressing on the acknowledgement button.
+
+1. Cancelling a pending no-motion alert
+
+ a. The device will start a “pending alarmâ€
+
+ b. Press the acknowledge button until the alarm pattern is silenced (approximately 1 second).
+ The device will return to regular operation.
+
+2. Silencing a no-motion alert
+
+ a. If you have not cancelled the alert within 30 seconds, the no-motion alert is communicated to 5555.
+
+ b. The device will trigger the “high alarm†pattern and will continue until the alert is cancelled or the battery runs out.
+
+ c. Press and hold the acknowledge button until the alarm pattern is silenced (approximately 3 seconds).
+ The device will return to regular operation.
+
+ d. <img src="img/pass_img-6.jpg"> Silencing an alarm will not cancel the call, 5555 will still intervene.
+
+### 4. Voice call alert
+
+1. When a phone call has been initiated by 5555, the device will trigger the “high alarm†pattern accompanied by a blue flashing of the LiveResponse light.
+
+2. To silence the alarm, press and hold the acknowledge button until the alarm is silenced (approximately 3 seconds).
+
+3. The LiveResponse confirmation light will continue to flash, signifying that an incoming call has been initiated.
+
+4. Once the call has been connected, the device will generate a long beep signifying the beginning of the two-way voice calling.
+
+5. In a noisy environment, it may be necessary to remove and hold the device near your face, as you would do with a two-way radio.
+
+6. Once the call is complete, the caller will end the call.
+The device will trigger a long beep signifying the end of the call.
+
+7. The device user cannot end the call.
+
+8. The LiveResponse confirmation light on the device will go off once the call has ended.
+
+9. NOTE: during the call, the volume of the speaker can be switched to speaker and back to lower volume by pressing the acknowledge button once.
+
+### 5. Low battery notification
+
+1. If the battery level on the device drops below 20% capacity, the device will flash the charging light and beep once every 5 minutes.
+Once the device battery is depleted, it will automatically shut down.
+
+2. This is considered an unsafe log off.
+If the device is configured to require a periodic check-in, a low battery shutdown will not stop the timer and a missed check-in alter will be generated.
+
+## 4. Types of alarms
+
+|Type|Beeper|Vibration|Visual indicator|
+|----|------|---------|----------------|
+|**Pending alarm**|Slow alarm pattern|Single repeating vibration|Alarm light array slow flash (red)|
+|**High alarm**| Fast alarm pattern| Double repeating vibration|Alarm light array fast flash (red)|
+|**Low battery**|Single beep once every 5 minutes|No vibration|Charge light, single flash once every 5 minutes|
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+---
+layout: page
+permalink: /external/lab/utilization-of-balances/
+shortcut: lab:utilization-of-balances
+redirect_from:
+ - /cards/lab:utilization-of-balances
+ - /external/cards/lab:utilization-of-balances
+---
+
+# Utilization of balances
+
+## Routine maintenance of analytical balances from Mettler-Toledo XS4002S and XPE205DR
+
+
+### 1. Personal protective equipment
+
+* Always wear a lab coat and gloves when using the balances.
+* If necessary, wear safety glasses.
+* If weighing dangerous, volatile compounds, use a balance located under a chemical hood.
+
+There are such balances on the 5th floor in BT1 and on the 2nd floor in BT2.
+
+### 2. Weighing
+
+**XPE205DR: used for 1.4 mg to 220 g**
+
+<div align="center">
+<img src="img/XPE205DR.jpg" height="400">
+</div>
+
+**XS4002S: used for 800 mg to 4.1 kg**
+
+<div align="center">
+<img src="img/XS4002S.jpg" height="400">
+</div>
+
+1. For XPE balances, check that the air bubble is in the middle of the circle.
+ It is located on the rear right foot of the instrument.
+ <div align="center">
+ <img src="img/XPE205DR_2.jpg" height="400">
+ </div>
+2. Switch on the balance by pressing on “On/Off†until the display appears.
+3. In order to allow an equilibration of electronics within the balance, allow it to stand for 10 to 15 minutes before starting using it.
+4. Zero the balance by pressing on “0â€.
+ a. For the XPE balance, make sure the doors are closed by pressing on “↨†or by passing your hand over the right and left upper parts of the terminal.
+5. Place an anti-static weighing boat or a weighing paper in the middle of the pan of the balance. Do not place the material to be weighted directly on the pan.
+6. If you weigh directly in a tube, place the Ergo Clip on the pan of the balance and place your tube in it.
+
+**Ergo clip**:
+
+<div align="center">
+<img src="img/Ergo-Clip_2.jpg" height="400">
+</div>
+
+7. Tare the balance by pressing on “Tâ€
+8. Place the material to be weighted in the anti-static weighing boat or on the weighing paper in the middle of the pan to avoid corner-load error, for the XPE balance,close the doors after loading your material.
+9. To prevent contamination of stock material, do not return unused substance to the stock bottle.
+10. After use, switch the balance off by pressing on the “ON/OFF†button.
+11. Contact the Quality Assistant if the logbook is full.
+12. If you notice anything wrong with the instrument, contact the instrument responsible.
+
+### 3. Cleaning
+
+1. After each use, the balance and the material used to weight have to be cleaned.
+2. **XPE205DR**:
+ * Open the doors and remove the weighing pan
+ * Wipe the surfaces with a brush
+ * Wipe the weighing pan and the inside of the chamber with a damp cloth
+ * Place the weighing pan back and make sure it is correctly placed
+3. **XS4002S**:
+ * Wipe the surfaces with a brush
+ * Wipe the weighing pan with a damp cloth
+ * If some powder fell off the pan, carefully remove the crown around the pan and clean it to remove any residue. If you need assistance, contact the instrument responsible person
+ <img src="img/Crown.jpg" height="400">
+ * When the crown is removed, carefully wipe with a damp cloth around the weighing pan
+ * Carefully place the crown back
+ * Always wipe the area around the balance with 70 % ethanol to remove any substance
+ * Spoons: wash them with a mild detergent and water
diff --git a/external/lab/utilization-of-pH-meter/description_pH-meters.jpg b/external/lab/utilization-of-pH-meter/description_pH-meters.jpg
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+version https://git-lfs.github.com/spec/v1
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diff --git a/external/lab/utilization-of-pH-meter/utilization-of-pH-meter.md b/external/lab/utilization-of-pH-meter/utilization-of-pH-meter.md
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index 0000000000000000000000000000000000000000..4c6c73fd7f7a7df6a37d7439894c4c65f5ebd578
--- /dev/null
+++ b/external/lab/utilization-of-pH-meter/utilization-of-pH-meter.md
@@ -0,0 +1,95 @@
+---
+layout: page
+permalink: /external/lab/utilization-of-pH-meter/
+shortcut: lab:utilization-of-pH-meter
+redirect_from:
+ - /cards/lab:utilization-of-pH-meter
+ - /external/cards/lab:utilization-of-pH-meter
+---
+
+# Utilization of pH meter
+
+## Routine Maintenance of pH meter “Seven Easy†and “Seven Compact†from Mettler-Toledo.
+
+## 1. Description
+
+<div align="center">
+<img src="img/../description_pH-meters.jpg" height="300">
+</div>
+
+## 2. Personal protective equipment
+
+Always wear a lab coat and gloves when working with the pH meter. Wear safety glasses if necessary.
+
+## 3. Prepare the calibration reagents
+
+1. The calibration cannot be performed using the reagents directly in their original containers, they need to be aliquoted.
+
+2. Transfer a few millilitres of each of the three calibration solutions in a 15 mL falcon tube. Label them with the pH value of the solution and the date of aliquot.
+3. Indicate the opening date on the original bottles and store them in the dark.
+
+4. Keep the aliquoted solutions for a month, then replace them with fresh ones.
+
+5. Indicate the date of exchange of the solutions in the log book.
+
+## 4. Calibration
+
+Calibration should be performed only if it has not been performed on the same day (see logbook) or if you have doubts about the calibration status of the instrument.
+The pH meter allows for calibration of 1, 2 or 3 points.
+The calibration standard solutions are of pH 4, 7 and 10.
+
+1. Rinse the electrode with deionized water, wipe carefully the outside of the electrode with a lint free tissue.
+
+2. Place the electrode in a calibration standard and press “CALâ€.
+
+3. The pH meter automatically endpoints when calibrating.
+
+4. For 2 points calibration, repeat steps 1 to 3 for the second calibration standard.
+
+5. For 3 points calibrations, repeat steps 1 to 3 with the third calibration standard.
+
+6. Rinse the electrode with deionized water, wipe carefully the outside of the electrode with a lint free tissue.
+
+7. After calibration, to return to sample measurement, press on:
+
+ a. “Read†for the Seven Easy
+ b. “Endâ€, then “Save†for the Seven Compact
+
+8. Add the calibration date in the equipment logbook.
+
+## 5. Measuring the pH
+
+1. pH is temperature sensitive, therefore, make sure that your solution is at the temperature you will use it (for example: 37°C for cell culture medium).
+
+2. Make sure the sample has been thoroughly mixed.
+
+ a. If you use magnetic stirrer, make sure you stop the stirrer before inserting the probe.
+
+3. Rinse the electrode with deionized water, wipe carefully the outside of the electrode with a lint free tissue.
+
+4. Place the electrode in the sample and press on “READ†to start the measurement.
+
+5. During measurement, the decimal point of the display flashes.
+
+6. Once the measurement is complete, the display freezes automatically.
+
+7. Rinse the electrode with deionized water and place it back in its storing solution container (a 15 mL falcon containing 5 mL of 3 M KCl for example).
+
+8. Adjust the pH of your solution, use a Pasteur glass pipet if you use strong acids or bases.
+
+## 6. Maintenance
+
+1. All calibration solutions should be kept away from the light.
+
+2. Any spillage has to be cleaned immediately. Use wipes to dry the spill, then clean with water.
+
+3. The electrode has to be maintained in 3 M KCl.
+
+ a. Do not allow it to dry.
+ b. Change the solution at least monthly.
+ c. Indicate the date of change in the log book.
+ d. If the filling solution is encrusted outside the electrode, remove it by rinsing the electrode with deionized water.
+
+4. Contact the quality assistant if the logbook is full.
+
+5. Would you notice any issue with the instrument, create a ticket.
diff --git a/index.md b/index.md
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--- a/index.md
+++ b/index.md
@@ -41,6 +41,12 @@ The How-to cards are intended to provide practical guidance in implementing Data
"icon": "<i class=\"large material-icons\">vpn_key</i>",
"link": "/cards#integrity"
},
+ {
+ "title": "Lab",
+ "caption": "I need information on wetlab equipment and procedures",
+ "icon": "<i class=\"large material-icons\">science</i>",
+ "link": "/cards#lab"
+ },
];
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