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Commit 707fbc85 authored by Shaman Narayanasamy's avatar Shaman Narayanasamy
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......@@ -56,6 +56,7 @@ RUN mkdir -p /home/imp/tmp /home/imp/lib \
&& mkdir -p /usr/db/trimmomatic \
&& cp adapters/* /usr/db/trimmomatic/. \
&& cd .. && rm -rf *rimmomatic*
## idba ud
RUN wget http://hku-idba.googlecode.com/files/idba-1.1.1.tar.gz \
&& tar -xzf idba-1.1.1.tar.gz \
......@@ -79,6 +80,7 @@ RUN wget http://hku-idba.googlecode.com/files/idba-1.1.1.tar.gz \
&& make \
&& mv bwa /usr/bin \
&& cd .. && rm -rf bwa*
## htqc toolbox
RUN wget http://downloads.sourceforge.net/project/htqc/htqc-1.91.1-Source.tar.gz \
&& tar -xzf htqc-1.91.1-Source.tar.gz \
......@@ -111,6 +113,7 @@ RUN wget http://downloads.sourceforge.net/project/htqc/htqc-1.91.1-Source.tar.gz
&& cp -r bin/* /usr/bin \
&& cp -r perl/* /etc/perl/. \
&& cd .. && rm -rf vcftools*
## prokka
RUN wget http://www.vicbioinformatics.com/prokka-1.10.tar.gz \
&& tar -xzf prokka-1.10.tar.gz \
......@@ -157,14 +160,30 @@ RUN echo "r <- getOption('repos'); r['CRAN'] <- 'http://cran.us.r-project.org';
&& Rscript -e "install.packages('beanplot')" \
&& Rscript -e "source('http://bioconductor.org/biocLite.R');biocLite('genomeIntervals')"
## Install KronaPlot
RUN cd /home/imp/lib \
&& wget "http://downloads.sourceforge.net/project/krona/KronaTools (Mac, Linux)/KronaTools-2.5.tar" \
&& tar -xvf KronaTools-2.5.tar \
&& cd KronaTools-2.5 \
&& perl install.pl
## htslib
RUN cd /tmp \
&& wget https://github.com/samtools/htslib/releases/download/1.2.1/htslib-1.2.1.tar.bz2 \
&& tar -jxvf htslib-1.2.1.tar.bz2 \
&& cd htslib-1.2.1 \
&& ./configure && make && make install \
&& cd .. && rm -rf htslib-1.2.1
## Platypus
RUN cd /home/imp/lib \
&& wget http://www.well.ox.ac.uk/bioinformatics/Software/Platypus-latest.tgz \
&& tar -xvzf Platypus-latest.tgz \
&& cd Platypus_0.7.9.1 \
&& cd Platypus_0.8.1 \
&& bash buildPlatypus.sh
## Megahit
RUN cd /home/imp/lib \
&& git clone https://github.com/voutcn/megahit.git \
......@@ -176,7 +195,6 @@ RUN cd /home/imp/lib \
######################
#COPY Snakefile /home/imp/integrated-metaomic-pipeline/Snakefile
#COPY src/ /home/imp/integrated-metaomic-pipeline/src
##RUN git clone http://yjarosz:gitlablcsb@lcsb-git.uni.lux/shaman/integrated-metaomic-pipeline.git
#################
# add test data #
......@@ -186,7 +204,7 @@ RUN cd /home/imp/lib \
######################
# runtime parameters #
######################
ENV PATH /home/imp/lib/trinityrnaseq_r20140717:/home/imp/lib/Platypus_0.7.9.1:/home/imp/lib/megahit:$PATH
ENV PATH /home/imp/lib/trinityrnaseq_r20140717:/home/imp/lib/Platypus_0.8.1:/home/imp/lib/megahit:$PATH
VOLUME ["/shared"]
WORKDIR /home/imp/integrated-metaomic-pipeline
......
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