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Commit 0ff85732 authored by Annegrät Daujeumont's avatar Annegrät Daujeumont Committed by Kaan Cimir
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Update external/lab-hsa/ppe/ppe.md Deleted external/lab-hsa/ppe/img/img1.png

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4 merge requests!544Update external/lab-hsa/ppe/ppe.md, external/lab-hsa/ppe/img/img1.png,...,!543Update external/lab-hsa/ppe/ppe.md, external/lab-hsa/ppe/img/img1.png,...,!526!459 conflict fix,!524[release] Regular merge of develop
external/lab-hsa/ppe/img/img1.png

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......@@ -19,7 +19,10 @@ Every LCSB employees, students and visitors are responsible
for wearing the approriate PPE that will vary depending on the hazards present in the room, but must always include:
- Appropriate lab coat
- Appropriate gloves (when handling biological materials or chemicals)
- Appropriate gloves (when handling biological materials or chemicals)
Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**
- Specific PPEs depending on the performed activity (faceshield, thermal gloves, themal appron,...)
It is highly recommended to also wear safety glasses or protective prescription glasses.
......@@ -102,6 +105,29 @@ Wear the glove above your lab coat.
<img src="img/img24.png">
</div>
### Work with strong chemical risk
Dealing with strong chemicals such as strong acids, bases,... requires specific gloves providing specific protection, on top of the regular PPEs.
Those gloves are not disposable gloves.
- **Chemical Gloves**
ANSELL: ChemTek 38-520
EN 374-1 Type A (A, B, C, I, K, L, N, P, S, T): permeation resistance of at least 30 minutes with at least six test chemical
EN 388 2010X: resistance to abrasion (0-4), resistance to cut (0-5), resistance to tear (0-4), resistance to perforation (0-4)
<div align="center">
<img src="img/img23.png" height="350">
<img src="img/img15.png" height="175">
<img src="img/img10.png" height="180">
<img src="img/img11.png" height="180">
<img src="img/img24.png">
</div>
### Autoclave unloading and other hot thermal risks
On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 gloves** located next to the autoclaves to protect you against hot thermal hazard. Those gloves are not disposable gloves.
......@@ -111,6 +137,7 @@ On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 g
<img src="img/img23.png" height="350">
<img src="img/img15.png" height="175">
<img src="img/img1.png">
<img src="img/img24.png">
</div>
......@@ -135,7 +162,7 @@ Those gloves are not disposable gloves.
<img src="img/img23.png" height="350">
<img src="img/img15.png" height="175">
<img src="img/img2.png">
<img src="img/img29.png">
<img src="img/img27.png" height="200">
</div>
### Distribution of Liquid Nitrogen in a Dewar
......@@ -158,18 +185,14 @@ Those gloves are not disposable gloves.
<div align="center">
<img src="img/img2.png">
<img src="img/img29.png">
<img src="img/img28.png">
<img src="img/img27.png" height="200">
<img src="img/img28.png" height="350">
</div>
## Usage of Liquid Nitrogen in open Dewars
### Usage of Liquid Nitrogen in open Dewars
Some experiments require to snap freeze the samples in Liquid Nitrogen or to keep samples frozen in Liquid Nitrogen during the manipulation. Having Liquid Nitrogen in an open Dewar on the bench top requires to wear on top of the regular PPEs:
- **Lab coat**: BSL1 or BSL2 depending on the activity
- **powder free nitiri
- **Cryo-gloves**
EN 420: general requirementse (identification, size, norms, composition,...)
......@@ -185,107 +208,43 @@ Those gloves are not disposable gloves.
<div align="center">
<img src="img/img23.png" height="350">
<img src="img/img24.png" height="350">
<img src="img/img25.png" height="350">
<img src="img/img15.png" height="175">
<img src="img/img2.png">
<img src="img/img29.png">
<img src="img/img27.png" height="200">
</div>
### Other cold thermal risks
Handling of samples in the -80°C freezers, in the cryostorage or in the cryopod
**Mandatory For:**
• Handling dry ice
• Low and ultra-low temperature freezers
• Automated cryostorage
• Sample storage in liquid Nitrogen
• Distribution of liquid nitrogen in a Dewar
### Lab coat
Wear a lab coat that covers your arms and torso when working with biological materials or chemicals.
Lab coats must be removed before leaving the laboratory and must be laundered regularly.
More informations about lab coat can be found in the dedicated [How-To card](https://howto.lcsb.uni.lu/external/lab-hsa/lab-coats/).
### Eye protection
Wear eye protection such as safety goggles or face shields when working with biological materials or chemicals.
Eye protection must fit snugly and not interfere with your vision.
### Gloves
Some activities performed in the lab are exposing your skin to various chemicals, infectious materials, high or low temperature.
To protect you, adequate gloves must be worn.
Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**
#### Gloves for specific activities
##### Thermal Risk - hot temperature
When handling samples in the -80°C freezers, in the -150°C freezer, in the cryopod or in dry-ice, it is recommended to wear, on top of the regular mandatory PPEs cotton white gloves to be worn under the regular nitrile glove. It will enable an improved dexterity compared to the regular cryo-gloves. Those cotton gloves are not disposable and should be kept in your lab coat.
<div align="center">
<img src="img/img23.png" height="350">
<img src="img/img25.png" height="350">
<img src="img/img6.png" width="225">
<img src="img/img15.png" height="175">
<img src="img/img24.png">
</div>
##### Thermal risk cold- cryogenic material handling
**Gloves to protect you against the risk of cold burns. Those gloves are not disposable gloves.**
----------------------------------------------------------
##### Thermal risk cold- samples or deliveries in dry ice handling
For samples or deliveries manipulation in dry ice or with the cryopod, instead of the classical cryogloves described above, white gloves to be worn under the regular nitrile glove can substituate the cryogloves. It will enable an improved dexterity.
<div align="center">
<img src="img/img5.png" width="225">
<img src="img/img6.png" width="225">
<img src="img/img7.png" width="225">
<img src="img/img8.png" width="225">
</div>
When using the cryopod, the hand is not in contact with liquid nitrogen.
In this particulate case, the cryogloves can be replaced by the cotton white gloves to be worn under the regular nitrogen gloves.
<div align="center">
<img src="img/img9.png">
</div>
##### Strong chemical risk
**Dealing with strong chemicals requires specific gloves providing specific protection.
Those gloves are not disposable gloves.
Use this type of glove each time you have to handle strong acids, bases,…**
Chemical Gloves
ANSELL: ChemTek 38-520
<div align="center">
<img src="img/img10.png" height="180">
<img src="img/img11.png" height="180">
</div>
Maximum protection against hazardous chemical (direct contact/strong corrosive).
Offers protection against aliphatic, halogenated and aromatic hydrocarbons as well as concentrated mineral acids.
**Recommended For:**
• Transferring liquids and solids between vessels and tanks and process equipment
• Unexpected leakages spills or other releases
##### Spill management
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