@@ -43,10 +43,6 @@ Contaminated sera and other filtration products have, in the past, been a major
## Mycoplasma detection
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There are two bacis testing methods for mycoplasma:
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@@ -58,9 +54,6 @@ Direct culture is the most effective and sensitive method for detecting mycoplas
There are a wide range of indirect test methods available for mycoplasma detection, including PCR-based kits, DNA fluorochome staining, autoradiography, ELISA, immunofluorescence, and specific biochemical assays. These tests are faster than direct culture and are often commercially available as kits.
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It is important to know what aspect of contamination the test is designed to detect, how well the test performs, its specificity (i.e., what strains of mycoplasma it detects and any likely causes of false positive or false negative reactions) and for detectable contamination, what level of sensitivity is achievable under the prescribed sampling and test conditions. Selection of test methods should be based on evaluation of the potential specificity and sensitivity of detection and the likelihood of inhibition of a positive result.
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@@ -77,10 +70,6 @@ Here are some examples of kits used by LCSB researchers:
