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Commit b361f3d2 authored by Laurent Heirendt's avatar Laurent Heirendt :airplane:
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Merge branch 'develop' into 'master'

[release] Regular merge of develop

See merge request !394
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2 merge requests!493update web IDE content,!394[release] Regular merge of develop
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......@@ -103,7 +103,7 @@ order: -1
<li><a href="{{ 'external/lab-equipment/maintenance_of_cold_traps' | relative_url }}">Maintenance of cold traps </a></li>
<li><a href="{{ 'external/lab-equipment/utilization-of-autoclaves' | relative_url }}">Utilization of autoclaves</a></li>
<li><a href="{{ 'external/lab-equipment/utilization-of-balances' | relative_url }}">Utilization of balances</a></li>
<li><a href="{{ 'external/lab-equipment/utilization-of-bsc' | relative_url }}">Utilization of Biosafety Cabinets (BSC)</a></li>
<li><a href="{{ 'external/lab-equipment/utilization-of-bsc' | relative_url }}">Biosafety Cabinets: good practices</a></li>
<li><a href="{{ 'external/lab-equipment/utilization-of-pH-meter' | relative_url }}">Utilization of pH meter</a></li>
</ul>
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......@@ -15,7 +15,7 @@ This is an overview on websites to manage work and help you with the navigation
| Link | Description |
| :------ | :------ |
|[www.uni.lu](https://www.uni.lu)|General website of the University. You can find general information as well as specific information on the faculties and institutes here.|
|[intranet.uni.lux](https://intranet.uni.lux)|The internal platform of the University. Here you find internal information and have access to different services such as the HR Employee Self-Service (ESS). By clicking on ‘My LCSB’ next to your name in the top right corner, you are directed to the LCSB Intranet with information on upcoming presentations, science tools and social events. **Please note:** This site can only be accessed when you are logged into the university network, either in your office or via a VPN client from elsewhere. See also the [corresponding How-to Card](https://howto.lcsb.uni.lu/?access:vpn-cerbere-access).|
|[uniluxembourg.sharepoint.com](https://uniluxembourg.sharepoint.com/) / [uniluxembourg.sharepoint.com/sites/lcsb/lscb_internal](https://uniluxembourg.sharepoint.com/sites/lcsb/lscb_internal/SitePages/default.aspx)|The internal platform of the University / the LCSB **Please note:** This site can only be accessed when you are logged into the university network, either in your office or via a VPN client from elsewhere. See also the [corresponding How-to Card](https://howto.lcsb.uni.lu/?access:vpn-cerbere-access).|
|[service.uni.lu](https://service.uni.lu/sp)|Here you find service and support from the university. You can create tickets on all regulatory and legal issues as well as on IT-related topics, e.g. to get access to specific folders and software.|
|[lums.uni.lu](https://lums.uni.lu)|The LUMS (LCSB User Management System) allows you to get access to internal resources such as software provided and/or hosted by the LCSB. **Please note:** You need to request an additional account to be able to log in as your usual UNI.LU-credentials will **not do the job.** The LUMS-accounts can also be created for external researchers and collaborators.|
|[howto.lcsb.uni.lu](https://howto.lcsb.uni.lu/)|This is where the How-to Cards are deposited and constantly updated. You can find answers on administrative as well as on lab-related procedures here. For complex instructions, a step-by-step tutorial with corresponding screenshots or examples is provided. We encourage you to bookmark this page as many answers to your questions might be covered by a card. If you have any suggestions for improvements on existing or additional How-to Cards, just contact the R3 department.|
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......@@ -8,38 +8,33 @@ redirect_from:
- /cards/lab:utilization-of-bsc
- /external/cards/lab:utilization-of-bsc
---
# Utilization of Biosafety Cabinets (BSC)
# Biosafety Cabinets: good practices
To ensure a good work quality in a sterile and safe environment, it is essential to know how to work in a BSC and what are the best practices.
Please check the following short videos from The World Health Organization (WHO).
1. **Introduction** to Biosafety Cabinets: <https://www.youtube.com/watch?v=KHCT9OJqxPo&t=1>
1. [Introduction](https://www.youtube.com/watch?v=KHCT9OJqxPo&t=1) to Biosafety Cabinets
2. **Preparation Steps** prior to working in a biosafety cabinet (BSC): <https://www.youtube.com/watch?v=4DoHJS8JL4U>
2. [Preparation Steps](https://www.youtube.com/watch?v=4DoHJS8JL4U) prior to working in a biosafety cabinet (BSC)
3. **Best Practices** for safe biosafety cabinet (BSC) usage: <https://www.youtube.com/watch?v=18QEJUA9XBs>
3. [Best Practices](https://www.youtube.com/watch?v=18QEJUA9XBs) for safe biosafety cabinet (BSC) usage
4. Biosafety cabinet (BSC) **Incident Management**: <https://www.youtube.com/watch?v=aS_TCZTCcsI>
4. Biosafety cabinet (BSC) [Incident Management](https://www.youtube.com/watch?v=aS_TCZTCcsI)
If you have any issue with a BSC, please send a [ticket](https://service.uni.lu/sp?id=sc_cat_item&sys_id=be0873f8db7070505c72ef3c0b96199f&sysparm_category=af924f17dbac38105c72ef3c0b96194b) to the Support Team.
If you are not sure a BSC is the right hood to use for your experiment, please check the How To Card related to the different types of hoods or contact the LCSB safety officer.
If you are not sure a BSC is the right hood to use for your experiment, please check the [How To Card](https://howto.lcsb.uni.lu/external/lab-equipment/hoods/) related to the different types of hoods or contact the LCSB safety officer.
If you have any question regarding the usage of a BSC, please send a ticket to the Support Team or contact the LCSB Safety Officer.
## Recommendations
## Good practices in a BSC
- The user must wear **lab coat** and **gloves** while using a BSC.
- The user must wear a BSL2 **lab coat** and **gloves** while using a BSC.
<div align="center">
<img src="img/image1.jpeg">
<img src="img/image2.jpg" width="161" high="190">
</div>
- The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
- Be aware that **UV-lights act only on surfaces exposed to them**, the surfaces in the shadow are not disinfected and a chemical disinfection is still necessary after a UV decontamination.
- The BSC must be **decontaminated before and after each use**. The interior surfaces should be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should **use Bacillol 30 foam or tissues**.
......@@ -49,6 +44,8 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
<img src="img/image4.jpeg">
</div>
- The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
Corrosive chemicals such as bleach should be avoided, but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels.
<div align="center">
......@@ -59,13 +56,13 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
- Your **hands** and all the **materials** that enter in the BSC must be **decontaminated with 70% ethanol or Bacillol 30 tissues**.
- Put **Incidin PLU**S in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
- Put **Incidin PLUS** in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
<div align="center">
<img src="img/image6.jpeg" width="166" high="267">
</div>
- Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium cross-contamination with other cell lines, ...).
- Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium, cross-contamination with other cell lines, ...).
- Set up workspace in a direction **from clean to dirty**.
......@@ -73,16 +70,34 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
<img src="img/image7.png">
</div>
- **Never cover the front or rear grills** with any material, even temporarily.
- **Never cover the front or rear grids** with any material, even temporarily.
- **Avoid movement of materials or excessive movement of hands and arms** through the front access opening during use. After entering allow the cabinet to stabilize before resuming work.
- **Avoid movement of materials or excessive movement of hands and arms** through the front access opening during use. After entering, allow the cabinet to stabilize before resuming work.
- Take care **not pass** with your hands **over open bottles, flasks or tip boxes**.
- When you open a flask or a medium, you can place the **lid with the opening up or down** on the bench, but make sure you put it **outside your working area**, at the back of the BSC.
- When you open a flask or a medium, you can place the **lid with the opening up or down** on the bench, but make sure you put it **outside your working area**, at the back of the BSC so you do not pass over it with your hands or arms.
- **Close open bottles, flask, falcons, tips boxes immediately after use**.
- **Remove your waste as soon as you have finish your work**.
- When work is completed, clear the workspace and clean the space with Ethanol 70% or with Bacillol 30. Please refer to the [Chemical and Biological Waste Management](https://howto.lcsb.uni.lu/external/lab-hsa/waste/).
## UV lights
Extract from Environmental Health and Safety, University of Washington: *The trouble with UV light in your biosafety cabinet*.
- **Close open bottles, flask, flacons, tips boxes immediately after use**.
You may want to turn on the UV lights. If so, be aware that it may not be as effective for sterilization/decontamination purposes as you need it to be.
Indeed, ultraviolet radiation is a form of non-ionizing radiation, and biological effects from it vary with wavelength, photon energy, and duration of exposure. The 100-280 nm wavelength band is designated as UV-C, which is used for germicidal purposes.
- **Don't store waste during the night in the BSC**.
The sterilization/decontamination activity of UV lights is limited by a number of factors, including:
- When work is completed, clear the workspace from clean and from contaminated materials. Please refer to the Waste Concept training <https://unisupport.docebosaas.com/learn/course/42/waste-management-concept>.
- **Penetration** In the dynamic air streams of BSCs, microorganisms beneath dust particles, plastics, and work surfaces are not affected by the UV light because it cannot penetrate particles so far from the UV source.
- **Relative humidity** The germicidal effects of UV light drop off precipitously when relative humidity is above 70%.
- **Temperature and air movement** The optimum temperature for the UV lamp to be effective is 25-27°C. Temperatures below this range result in reduced efficacy, and air movement can exacerbate this.
- **Cleanliness** Dust and dirt block the germicidal effectiveness of the UV lamp, so weekly cleanings are necessary.
- **Age** Check UV lamps every six months to assure proper function, as the amount of germicidal wavelength emitted decreases with bulb age and hours of use.
- **Overuse** UV lights are routinely left on overnight or longer in an effort to decontaminate workspaces, but this practice can result in the germicidal wavelength no longer being produced by the bulb.
For these reasons and environmental concerns, it is not recommend to use the UV lights in BSCs. Retrofitting any equipment (e.g., UV lights) into a cabinet may alter the air flow characteristics, invalidate the manufacturer warranty, and is not recommended.
......@@ -25,25 +25,31 @@ The LCSB regularly welcomes newcomers who have just joined one of the research g
## 1. To do as a team assistant
The [team assisstants](https://howto.lcsb.uni.lu/?general:secretariat) support the scientists to navigate through the administrative environment at the LCSB. To make sure the newcomer feels comfortable in the new group, the team assistant takes care of the following procedures:
- Align with PI to find a godparent.
- Align with PI which PC is foreseen for the newcomer: either using an old spare one or ordering a new one.
* Spare PC: request a reinstallation from the IT department ([open a ticket](https://service.uni.lu/sp?id=sc_cat_item&sys_id=ead9a1ecdbb358102fa838aa7c9619df&sysparm_category=c41b4681db9f10502fa838aa7c961990)).
* New PC: budget code (OTP) needs to be specified, [request an offer from IT](https://service.uni.lu/sp?id=sc_cat_item&sys_id=5ea27df9dbbb50508bcbf9b41d96195f&sysparm_category=c41b4681db9f10502fa838aa7c961990): specify type of keyboard (Quertz, Querty UK/US, Azerty) and accessories like mouse, adapters, etc., create PO and place the order. Keep in mind that this process needs up to 8 weeks.
- Align with PI to define a desk for the newcomer and update [Archibus](https://archibus.uni.lux/archibus/) accordingly.
- Request a badge and the necessary office access in [Archibus](https://archibus.uni.lux/archibus/) (lab access is granted by the lab support team accordingly).
- Open tickets to
* Order a [phone and a phone extension](https://service.uni.lu/sp?id=sc_cat_item&sys_id=9f11411adbd69c902fa838aa7c961959&sysparm_category=1679cecddb5f10502fa838aa7c9619ab) attributed to the newcomer
* Add newcomer to [mail lists](https://service.uni.lu/sp?id=sc_cat_item&sys_id=b7b07412db969c902fa838aa7c9619ca&sysparm_category=0784418e1b312450ff1c8739cd4bcb44) (e.g., LCSB-team, group list).
* Get access to the [intranet](https://service.uni.lu/sp?id=sc_cat_item&sys_id=448026d8dbd07450c542f9b2f39619c6&sysparm_category=0784418e1b312450ff1c8739cd4bcb44&catalog_id=26a236a5dbd2d8902fa838aa7c961948), [shared folders](https://service.uni.lu/sp?id=sc_cat_item&sys_id=d8c9740e1bfde050ff1c8739cd4bcb94) on the ATLAS server, LUMS [account](https://service.uni.lu/sp?id=sc_cat_item&sys_id=c536257ddb336010ca53454039961936&sysparm_category=6b76697ddb336010ca534540399619f8), etc.
- Prepare a welcome bag with office supplies pens, notebook, scissors, stapler, ruler etc. and give it to the newcomer on the first day.
- Meet with newcomer to explain the different administrative procedures and existing tools such as travel organization, TAR, PO, expense reports, the Service Now ticket system, HR.
- Order business cards via [Web-to-Print](https://www.webtoprint.lu/uni/UserEditFormFilling.aspx) if needed.
- Align with PI to find a godparent.
- Align with PI which PC is foreseen for the newcomer: either using an old spare one or ordering a new one.
* Spare PC: request a reinstallation from the IT department ([open a ticket](https://service.uni.lu/sp?id=sc_cat_item&sys_id=ead9a1ecdbb358102fa838aa7c9619df&sysparm_category=c41b4681db9f10502fa838aa7c961990)).
* New PC: budget code (OTP) needs to be specified, [request an offer from IT](https://service.uni.lu/sp?id=sc_cat_item&sys_id=5ea27df9dbbb50508bcbf9b41d96195f&sysparm_category=c41b4681db9f10502fa838aa7c961990): specify type of keyboard (Qwertz, Qwerty UK/US, Azerty) and accessories like mouse, adapters, etc., create PO and place the order. Keep in mind that this process needs up to 8 weeks.
- Align with PI to define a desk for the newcomer and update [Archibus](https://archibus.uni.lux/archibus/) accordingly.
- Request a badge and the necessary office access in [Archibus](https://archibus.uni.lux/archibus/) (lab access is granted by the lab support team accordingly).
- Open tickets to
* Order a [phone and a phone extension](https://service.uni.lu/sp?id=sc_cat_item&sys_id=9f11411adbd69c902fa838aa7c961959&sysparm_category=1679cecddb5f10502fa838aa7c9619ab) attributed to the newcomer
* Add newcomer to [mail lists](https://service.uni.lu/sp?id=sc_cat_item&sys_id=b7b07412db969c902fa838aa7c9619ca&sysparm_category=0784418e1b312450ff1c8739cd4bcb44) (e.g., LCSB-team, group list).
* Get access to the [intranet](https://service.uni.lu/sp?id=sc_cat_item&sys_id=448026d8dbd07450c542f9b2f39619c6&sysparm_category=0784418e1b312450ff1c8739cd4bcb44&catalog_id=26a236a5dbd2d8902fa838aa7c961948), [shared folders](https://service.uni.lu/sp?id=sc_cat_item&sys_id=d8c9740e1bfde050ff1c8739cd4bcb94) on the ATLAS server, LUMS [account](https://service.uni.lu/sp?id=sc_cat_item&sys_id=c536257ddb336010ca53454039961936&sysparm_category=6b76697ddb336010ca534540399619f8), etc.
- Prepare a welcome bag with office supplies pens, notebook, scissors, stapler, ruler etc. and give it to the newcomer on the first day.
- Meet with newcomer to explain the different administrative procedures and existing tools such as travel organization, TAR, PO, expense reports, the Service Now ticket system, HR.
- Order business cards via [Web-to-Print](https://www.webtoprint.lu/uni/UserEditFormFilling.aspx) if needed.
## 2. To do as a godparent
When a new member joins the LCSB, the group PI is asked to nominate a senior member of the group as “godparent” who guides the newcomer during the on-boarding process. The godparent is showing the newcomer around the LCSB premises (BT1, BT2, Ketterthill, Roudeneck/BTL and MNO) and introduce the newcomer to the team and to the relevant LCSB staff (e.g., communication (BT2), financial (Ketterthill) and grants (MNO) teams, support biotech and the team assistant, and takes care of some of the early procedures:
- Open tickets to
* Pick up the computer on the first day of the newcomer
* Pick up the entrance badge together with the newcomer at the Security office in MSA 1st floor
* Pick up the computer on the first day of the newcomer
* Pick up the entrance badge together with the newcomer at the Security office in MSA 1st floor
- Show how to get office supplies (communication office in BT2).
- Explain Uni/LCSB [intranet](https://uniluxembourg.sharepoint.com/sites/the_university), newsletter, services and [important links](https://howto.lcsb.uni.lu/?general:links).
- Explain the lunch voucher system and where to get food.
......@@ -54,6 +60,7 @@ When a new member joins the LCSB, the group PI is asked to nominate a senior mem
- Encourage the newcomer takes an appointment for the Uni photo shoot (Newcomer should get an email).
## 3. To do as a newcomer
- Get acquainted with your colleagues, especially your group, the group secretary, and research support teams (ask your godparent to introduce you).
- Familiarise yourself with different LCSB tools e.g., ATLAS server, LUMS account, owncloud, the regular meetings (LCSB team meeting (mandatory), TGIaF, group meetings, CETs if you are a uni.lu PhD candidate), the lunch vouchers distribution, etc.
- Uni/LCSB policies and culture: how to find details about them on the [intranet](https://uniluxembourg.sharepoint.com/) and [How-to Cards](https://howto.lcsb.uni.lu/), and [important links](https://howto.lcsb.uni.lu/?general:links)
......@@ -65,6 +72,7 @@ When a new member joins the LCSB, the group PI is asked to nominate a senior mem
- Take an appointment for the Uni photo shoot (you should get an email).
## 4. Welcome meetings
- **University Welcome Day**: The newcomers must attend the University Welcome Day to get a general overview of the university procedures and policies. You should get an invitation from HR Administration. The presenter will explain you, where to store and find personal & general information and to understand globally the UL's tools for their daily requests: Atlas Server, Ticket system, HR4U, Fiori platform, Intranet, Archibus and more. In addition, you will get general information on the medical examination, social security, child allowances and renumeration.
- **LCSB Welcome meeting**: Once a month a two hours meeting is organised at the LCSB for the members who arrived recently. During the first part, a round table allow participants to introduce themselves and to place their “location sticker” on the LCSB map in BT2 staircase. Then, a member of the communication team presents the LCSB, its goals and values, and provides some practical information (who’s who, basic procedures, where to find answers, etc.). The second part is dedicated to security and safety (building evacuation, emergency exits, lab safety…).
The participants also get a copy of the LCSB annual and research reports and the slides used during the meeting are shared. The newcomers receive a calendar invitation for this meeting, sent by the communication team.
......@@ -72,11 +80,11 @@ The participants also get a copy of the LCSB annual and research reports and the
In order to introduce the newcomers to the whole LCSB team, the new members are mentioned in the LCSB newsletter (Photo, name, position and email address in the “Newcomers” section). They can also introduce themselves during the weekly team meeting at the LCSB.
## 5. Services and websites
You will need two accounts to make use of services and tools:
* **UNI-LU account:** To use the IT services, also known as SIU, will give you your account at your first working day
* **LUMS account:** This account is used for particular services (see below) and will be created after you have your UNI-LU account. Your godparent can assist you in that.
#### The **UNI-LU account** is used to access the following services:
* Mail services, including web mail accessible from the browser via: [https://owa.uni.lu](https://owa.uni.lu)
......@@ -85,7 +93,7 @@ You will need two accounts to make use of services and tools:
* SAP, to make leave requests, see and print payslips and other HR-related services: [https://fiori.uni.lu/fiori](https://fiori.uni.lu/fiori)
* Webex which is used for all virtual work meetings (implemented due to the COVID-19 pandemic), unless the other party requires the use of other software. You can login with UNI-LU account at: [https://unilu.webex.com/](https://unilu.webex.com/)
* University Intranet: [https://uniluxembourg.sharepoint.com/](https://uniluxembourg.sharepoint.com/)
* LCSB Intranet: [https://intranet.uni.lux/the_university/lcsb/lscb_internal/Pages/default.aspx](https://intranet.uni.lux/the_university/lcsb/lscb_internal/Pages/default.aspx)
* LCSB Intranet: [uniluxembourg.sharepoint.com/sites/lcsb/lscb_internal](https://uniluxembourg.sharepoint.com/sites/lcsb/lscb_internal/SitePages/default.aspx)
* specific communication channels, such as Slack, Basecamp an others, depending on your team. Your godparent will provide you with further information.
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