@@ -22,8 +22,11 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
- The user must wear **lab coat** and **gloves** while using a BSC.
<imgsrc="img/image1.jpeg"width="40">
<imgsrc="img/image2-jpeg"width="40">
<divalign="center">
<imgsrc="img/image1.jpeg">
<imgsrc="img/image2.jpg"width="161"high="190">
</div>
- The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
...
...
@@ -31,12 +34,16 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
- The BSC must be **decontaminated before and after each use**. The interior surfaces should be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should **use Bacillol 30 foam or tissues**.
<divalign="center">
<imgsrc="img/image3.jpeg">
<imgsrc="img/image4.jpeg">
</div>
Corrosive chemicals such as bleach should be avoided, but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels.
<divalign="center">
<imgsrc="img/image5.png">
</div>
- Allow the cabinet to run for at least **10 minutes** after switching on.
...
...
@@ -44,13 +51,17 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
- Put **Incidin PLU**S in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
<imgsrc="img/image6.jpeg">
<divalign="center">
<imgsrc="img/image6.jpeg"width="166"high="267">
</div>
- Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium cross-contamination with other cell lines, ...).
- Set up workspace in a direction **from clean to dirty**.
<divalign="center">
<imgsrc="img/image7.png">
</div>
-**Never cover the front or rear grills** with any material, even temporarily.