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p6_gas-network-total-failure-backup-plan_bt1-2/p6_gas-network-total-failure-backup-plan_bt1-2.md

+---
+layout: page
+permalink: /internal/lab/p6_gas-network-total-failure-backup-plan_bt1-2/
+---
+
+# P6: Gas network total failure backup plan BT1/BT2
+
+## CO2  
+This process covers the actions to take in case of total failure of the carbon dioxide (CO2, dioxyde de carbone) gas network due to pipeline infrastructure breakdown.  
+
+1. Check the CO2 connection at the back of each incubator and at the connection of the manometer to identify a possible leak.
+To do so, use the leak detector spray or the gas leak detector device.  
+
+2.	Also investigate the LSRU floors  
+
+3.	If a leak can be identified, fix it:
+
+- plug the tubing back
+- close the manometer connecting the incubator to the network (see arrow on the following picture): transfer the cells to another incubator  
+
+<div align="center">
+<img src="p6_img_1.png">
+</div>  
+
+4. If there no leak is detected, call 5555 and ask them to contact the SIL duty call.  
+ 
+5. Notify the LCSB duty call manager and their backup by email, fill and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en)  
+
+## N2  
+
+This process covers the actions to take in case of total failure of the nitrogen (N2, azote) gas network  due to pipeline infrastructure breakdown.
+It consists in the rescue of glove boxes and hypoxic incubators by connecting them to bottles of nitrogen.  
+
+1. Identify the pipeline infrastructure breakdown:  
+
+- Check the “gas bottle exchange record sheet” (Appendix 03) located on the door inside the gas deposit room.  
+- If the N2 bottles have been changed in the last 48 h, this is indicative of a pipeline infrastructure break down.  
+
+2. Check the N2 connection at the back of each glovebox and hypoxic incubator and at the connection of the manometer to identify a possible leak.
+To do so, use the leak detector spray or the gas leak detector device.  
+
+3. Also investigate the LSRU floors  
+4. If a leak can be identified, fix it:  
+
+- plug the tubing back  
+- close the manometer, do not transfer the samples  
+
+5. If there no leak is detected, call 5555 and ask them to contact the SIL duty call.  
+6. Notify the LCSB duty call manager and their backup by email, fill and fill the [Duty call report form](https://ulsurvey.uni.lu/index.php/936567?lang=en) 
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