Update external/lab-equipment/utilization-of-bsc/utilization-of-bsc.md

external/lab-equipment/utilization-of-bsc/utilization-of-bsc.md

@@ -8,7 +8,7 @@ redirect_from:
   - /cards/lab:utilization-of-bsc
   - /external/cards/lab:utilization-of-bsc
 ---
-# Utilization of Biosafety Cabinets (BSC)
+# Biosafety Cabinets: good practices
 
 To ensure a good work quality in a sterile and safe environment, it is essential to know how to work in a BSC and what are the best practices.
 
@@ -28,18 +28,15 @@ If you are not sure a BSC is the right hood to use for your experiment, please c
 
 If you have any question regarding the usage of a BSC, please send a ticket to the Support Team or contact the LCSB Safety Officer.
 
-## Good practices in a BSC
+## Good practices
 
--   The user must wear **lab coat** and **gloves** while using a BSC.
+-   The user must wear a BSL2 **lab coat** and **gloves** while using a BSC.
 
 <div align="center">
 <img src="img/image1.jpeg">
 <img src="img/image2.jpg" width="161" high="190">
 </div>
 
-
--   The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
-
 -   Be aware that **UV-lights act only on surfaces exposed to them**, the surfaces in the shadow are not disinfected and a chemical disinfection is still necessary after a UV decontamination.
 
 -   The BSC must be **decontaminated before and after each use**. The interior surfaces should be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should **use Bacillol 30 foam or tissues**.
@@ -49,6 +46,8 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
 <img src="img/image4.jpeg">
 </div>
 
+-   The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
+
 Corrosive chemicals such as bleach should be avoided, but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels.
 
 <div align="center">
@@ -59,13 +58,13 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
 
 -   Your **hands** and all the **materials** that enter in the BSC must be **decontaminated with 70% ethanol or Bacillol 30 tissues**.
 
--   Put **Incidin PLU**S in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
+-   Put **Incidin PLUS** in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
 
 <div align="center">
 <img src="img/image6.jpeg" width="166" high="267">
 </div>
 
--   Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium cross-contamination with other cell lines, ...).
+-   Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium, cross-contamination with other cell lines, ...).
 
 -   Set up workspace in a direction **from clean to dirty**.
 
@@ -73,16 +72,18 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
 <img src="img/image7.png">
 </div>
 
--   **Never cover the front or rear grills** with any material, even temporarily.
+-   **Never cover the front or rear grids** with any material, even temporarily.
 
--   **Avoid movement of materials or excessive movement of hands and arms** through the front access opening during use. After entering allow the cabinet to stabilize before resuming work.
+-   **Avoid movement of materials or excessive movement of hands and arms** through the front access opening during use. After entering, allow the cabinet to stabilize before resuming work.
 
 -   Take care **not pass** with your hands **over open bottles, flasks or tip boxes**.
 
--   When you open a flask or a medium, you can place the **lid with the opening up or down** on the bench, but make sure you put it **outside your working area**, at the back of the BSC.
+-   When you open a flask or a medium, you can place the **lid with the opening up or down** on the bench, but make sure you put it **outside your working area**, at the back of the BSC so you do not pass over it with your hands or arms.
+
+-   **Close open bottles, flask, falcons, tips boxes immediately after use**.
 
--   **Close open bottles, flask, flacons, tips boxes immediately after use**.
+-   **Remove your waste as soon as you have finish your work**.
 
--   **Don't store waste during the night in the BSC**.
+-   When work is completed, clear the workspace and clean the space with Ethanol 70% or with Bacillol 30. Please refer to the Waste Concept training <https://unisupport.docebosaas.com/learn/course/42/waste-management-concept>.
 
--   When work is completed, clear the workspace from clean and from contaminated materials. Please refer to the Waste Concept training <https://unisupport.docebosaas.com/learn/course/42/waste-management-concept>.
+- You may want to turn on the UV lights.