@@ -37,19 +37,23 @@ The number one source of mycoplasma contamination is other infected cell lines.
The second common source is the cell culturist; good training especially in aseptic techniques combined with the strategic use of a tested cell repository will greatly reduce the opportunities for contamination via this route. Talking and sneezing can generate significant amounts of aerosols that have been shown to contain mycoplasma. Also diry lab coats can be a source of contamination when a dust-laden sleeve is put into a laminar flow hood and dust particles fall into cultures.
Contaminated sera and other filtration products have been in the past a major source of contamination, but nowadays they only play a minor role in mycoplasma contaminations.
Contaminated sera and other filtration products have, in the past, been a major source of contamination, but nowadays they only play a minor role in mycoplasma contaminations.
## Mycoplasma detection
There are two bacis testing methods for mycoplasma:
1. Direct culture in media
Direct culture is the most effective and sensitive method for detecting mycoplasma, but it is also the most difficult and time-consuming.
2. Indirect tests that measure specific characteristics of mycoplasma
There are a wide range of indirect test methods available for mycoplasma detection, including PCR-based kits, DNA fluorochome staining, autoradiography, ELISA, immunofluorescence, and specific biochemical assays. These tests are faster than direct culture and are often commercially available as kits.
### Mycoplasma Control Kits
There are several methods for checking whether you have mycoplasma contaminations.
They include .....
### Mycoplasma Control Kits
The easiest way to do it, is to use a mycoplasma detection kit.
If you are not sure what test methods or kit to use, please do not hesitate your collegues at LCSB.
Here are some examples of kits used by LCSB researchers:
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@@ -57,4 +61,7 @@ Here are some examples of kits used by LCSB researchers:
