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# Personnal Protective Equipment (PPE)

**First of all, before entering the laboratories**

Tie back long hair and avoid wearing jewelry that could come into contact with biological materials/chemicals or damage the gloves. 

If possible avoid to wear contact lenses, if not else possible wear eye protection over them.

Closed-toe shoes are required in the laboratory.

## PPE Requirements

### Lab coat

Wear a lab coat that covers your arms and torso when working with biological materials or chemicals. 

Lab coats must be removed before leaving the laboratory and must be laundered regularly. 

More informations about lab coat can be found in the dedicated [How-To card](https://howto.lcsb.uni.lu/external/lab-hsa/lab-coats/).

### Eye protection

Wear eye protection such as safety goggles or face shields when working with biological materials or chemicals. 

Eye protection must fit snugly and not interfere with your vision. 

### Gloves

Some activities performed in the lab are exposing your skin to various chemicals, infectious materials, high or low temperature. 
To protect you, adequate gloves must be worn. 

Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**

#### Gloves for specific activities

##### Thermal Risk - hot temperature

**Gloves to be used to protect you against thermal hazard. Those gloves are not disposable gloves.**

Ansell Gladiator 16-650

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**Recommended For:**

•	Handling material coming from the autoclave

**Properties:**

•	Temperature resistance (250°C)

•	Good cut resistence

•	Very good wet & dry grip

##### Thermal risk cold- cryogenic material handling

**Gloves to protect you against the risk of cold burns. Those gloves are not disposable gloves.**

Cryo-Gloves

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**Mandatory For:**

•	Handling dry ice

•	Low and ultra-low temperature freezers

•	Automated cryostorage

•	Sample storage in liquid Nitrogen

•	Distribution of liquid nitrogen in a Dewar

##### Thermal risk cold- samples or deliveries in dry ice handling

For samples or deliveries manipulation in dry ice or with the cryopod, instead of the classical cryogloves described above, white gloves to be worn under the regular nitrile glove can substituate the cryogloves. It will enable an improved dexterity.

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When using the cryopod, the hand is not in contact with liquid nitrogen. 
In this particulate case, the cryogloves can be replaced by the cotton white gloves to be worn under the regular nitrogen gloves.

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##### Strong chemical risk

**Dealing with strong chemicals requires specific gloves providing specific protection. 
Those gloves are not disposable gloves. 
Use this type of glove each time you have to handle strong acids, bases,…**

Chemical Gloves

ANSELL: ChemTek 38-520

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Maximum protection against hazardous chemical (direct contact/strong corrosive).
Offers protection against aliphatic, halogenated and aromatic hydrocarbons as well as concentrated mineral acids.

**Recommended For:**

•	Transferring liquids and solids between vessels and tanks and process equipment

•	Unexpected leakages spills or other releases

##### Spill management

**Spill management requires to protect the skin against two type of risks: the chemicals and the glass/plasticware debris. For this purpose, use the gloves provided in the spill kit.**

ANSELL: AlphaTec 58-735

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