Merge branch 'sh-bsc' into 'develop'

 Utilization of BSC

See merge request !329

external/lab/utilization-of-bsc/utilization-of-bsc.md

+---
+layout: page
+permalink: /external/lab/utilization-of-bsc/
+shortcut: lab:dishwasher-utilization-of-bsc
+redirect_from:
+  - /cards/lab:dishwasher-utilization-of-bsc
+  - /external/cards/lab:dishwasher-utilization-of-bsc
+---
+
+# Utilization of BSC
+
+**Biosafety cabinet (BSC)** are designed to protect the operator, the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating substances containing infectious agents, such as viruses, bacteria and primary tissue cultures. Unlike a chemical hood, a BSC doesn’t protect the operator from volatile toxic chemicals. At LCSB, the BSC are identified with a sticker:
+
+<div align="center">
+<img src="img/img1.png" width="500">
+</div>
+
+Basically, the inward air flow from the room forms an air curtain and minimizes the escape of aerosols from the BSC which provides operator protection. The filtered downward air flow protects the samples and the work inside the BSC. About 70% of the air is internally recirculated in the laminar-flow pattern via the main HEPA filter and about 30% of the air recirculated into the room via a second HEPA filter.
+
+<div align="center">
+<img src="img/img2.png" width="350">
+</div>
+
+The World Health Organization (WHO) published short videos on how to properly use a BSC, take a few min to watch them, they are quite short but full of interesting informations:
+1.	[Introduction to Biosafety Cabinets](https://www.youtube.com/watch?v=KHCT9OJqxPo&t=1)
+2.	[Preparation Steps Prior to Working in a Biosafety Cabinet (BSC)](https://www.youtube.com/watch?v=4DoHJS8JL4U)
+3.	[Best Practices for Safe Biosafety Cabinet (BSC) Usage](https://www.youtube.com/watch?v=18QEJUA9XBs)
+4.	[Biosafety Cabinet (BSC) Incident Management](https://www.youtube.com/watch?v=aS_TCZTCcsI)
+
+Carefully read the below section, it contains specific information for LCSB equipment and practices: how to operate our BSC, how to deal with a biological spill, how to use specific BSC (KOJAIR, LAMSYSTEMS, BIOAIR), how to maintain a BSC and how to report an issue with a BSC.
+
+## Good practices in a BSC
+-	The user must wear **lab coat** and **gloves** while using a BSC (see [Lab coats](https://howto.lcsb.uni.lu/external/lab/lab-coats/))
+-	The BSC should be empty when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC
+-	Be aware that UV-lights act only on surfaces exposed to them, the surfaces in the shadow are not disinfected (ex: below a box, behind a pipette,...). Due to their strong limitation, UV do not replace a chemical disinfection that is still necessary when UV are used. Because of this, we do not recommend to use UV lights as they have strong limitations and give a false sense of safety
+-	**The BSC must be decontaminated before and after each use**. The interior surfaces should be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should use Bacillol 30 foam or tissues:
+<div align="center">
+<img src="img/img3.png" width="250">
+</div>
+Corrosive chemicals such as bleach should be avoided (bleach corrodes stainless steel), but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels
+<div align="center">
+<img src="img/img4.png" width="250">
+</div>
+
+-	Before starting to work, or switching the BSC OFF, allow the cabinet to run for at least 15 minutes. It allows several air filtration to be performed
+-	Your hands and all the materials that enter in the BSC must be decontaminated with 70% ethanol or Bacillol 30 tissues
+-	Put Incidin PLUS in the aspiration pump and disinfect the pump tubing:
+<div align="center">
+<img src="img/img5.png" width="100">
+</div>
+
+-	Control the cells in the microscope before starting to work to detect contamination in your cell culture (change in the medium color, cloudiness of the medium, cross-contamination with other cell lines, …)
+-	Set up workspace in a direction from clean to dirty:
+<div align="center">
+<img src="img/img6.png" width="350">
+</div>
+
+-	Never cover the front or rear grills with any material, even temporarily
+-	Avoid movement of materials or excessive movement of hands and arms through the front access opening during use. When you enter or exit the cabinet do so in a straight on fashion. After entering allow the cabinet to stabilize before resuming work
+-	Take care not pass with your hands over open bottles, flasks or tip boxes
+-	When you open a flask or a medium, you can place the lid with the opening up or down on the bench, but make sure you put it outside your working area, at the back of the BSC
+-	Close open bottles, flask, flacons, tips boxes immediately after use
+-	Don’t store waste during the night in the BSC
+-	When work is completed, clear the workspace from clean and contaminated materials (papers can be discarded in blue bag bins, contaminated material into infectious waste, and cell culture liquids can be aspirated with a pump)
+
+Infectious waste:
+<div align="center">
+<img src="img/img7.png" width="150">
+</div>
+
+-	Recycling baskets are found on each floor to discard plastics, empty tip boxes and empty plastic medium bottles **without antibiotics** (if presence of antibiotics, rinse 1X and autoclave the rinsing liquid). Filter bottles have to be autoclaved and therefore should not be disposed as recyclable material
+<div align="center">
+<img src="img/img8.png" width="600">
+</div>
+
+
+## How to deal with a spill in a BSC
+
+### **Minor spill in a BSC (few milliliters of hazardous biological material inside a BSC)**
+The main challenge in this case is to keep benefiting from the protection offered by the BSC while avoiding to export any contamination outside the cabinet. 
+-	Keep the BSC running and keep applying the rules for safe work in a BSC
+-	If your gloves have been exposed to contamination, change gloves
+-	Close all open containers
+-	Spray gently the spilled area and its surroundings with 70% ethanol
+-	Wipe the work surface and sprayed objects with some absorbing paper
+-	Discard the absorbing paper, gloves and everything that needs to be eliminated in the infectious waste
+-	Put on new gloves
+-	Proceed to a routine decontamination of the BSC using 70% ethanol or the disinfectant that is used for routine decontamination
+-	Resume or terminate work session
+-	Notify the incident to your laboratory safety coordinator
+
+### **Large spill in a BSC (more than few milliliters or more complicated spill situations)**
+The main challenge in this case is also to keep benefiting from the protection offered by the BSC while avoiding to export any contamination outside the cabinet. In case the spill would not be contained in the BSC, priority should be given to treat the part of the spill that lays outside the cabinet while keeping the BSC running.
+-	Keep the BSC running and keep applying the rules for safe work in a BSC
+-	If your gloves have been exposed to contamination, change gloves
+-	Close all open containers
+-	Cover the spilled liquids with absorbing paper
+-	Pour gently the absorbing paper with 70% ethanol in order to have it soaked
+-	Let the disinfecting solution act for 30 minutes, leaving all contaminated material within the BSC
+-	In the meantime, wash hands with soap and prepare other materials that would be needed (e.g., scoop, scraper, forceps, sharp safety box…)
+-	With new gloves on, clean up the BSC and remove all debris and the absorbing material from the BSC and dispose them carefully in the waste bag or container for contaminated waste
+-	Spray gently the exposed surfaces inside the BSC with 70% ethanol
+-	Wipe surfaces and sprayed objects with absorbing paper
+-	Discard all the materials in the waste bag located inside the BSC, then discard the bag in the external waste bag or container
+-	Change gloves
+-	Proceed to a final decontamination of the BSC with the disinfectant that is used in routine
+-	Resume or terminate work session
+-	Notify the incident to your laboratory safety coordinator
+
+### **Spill outside a BSC**
+This procedure covers liquid spills that are not likely to provoke important aerosols (low energy spills such as leakage from a non-pressurized equipment), occurring on an open bench or on the ground.
+-	Alert people around
+-	Change gloves as well as lab coat if they have been exposed to the spill
+-	Secure the spillage area in order to prevent others to use it (bench) or walk in it (floor)
+-	Prepare spill kit
+-	Spread Haz-Tab granules on the spill and cover with absorbing material
+-	Let the disinfectant act for 15 minutes
+-	Clean up the area, using the scoop, scraper and, if needed, resistant gloves to collect broken glass
+-	Discard the absorbing material, gloves and everything that needs to be eliminated in infectious waste
+-	Spray the spilled area and its surroundings (exposed parts of furniture, equipment and walls) widely with 70% ethanol
+-	Let the disinfectant act for 5 minutes
+-	Wipe exposed surfaces with absorbing material
+-	Proceed to a last surface decontamination of the area wiping it with a freshly diluted Haz-Tab solution (concentration of available chlorine from 1,5 g/l in clean conditions to 6 g/l in dirty conditions)
+-	Notify the incident to your laboratory safety coordinator
+
+## How to use KOJAIR BSC
+<div align="center">
+<img src="img/img9.png" width="200">
+</div>
+
+Control panel:
+<div align="center">
+<img src="img/img10.png" width="500">
+</div>
+
+**Turning on/off the cabinet**
+
+-	If the power is off, turn the key that is located on the left side of the control panel
+<div align="center">
+<img src="img/img11.png" width="150">
+</div>
+
+-	Place the sash in working position by pressing on <img src="img/img12.png" width="30">
+
+-	To start the ventilation, press on <img src="img/img13.png" width="30">  until the screen displays "*WAIT*". The message “*Kojair Normal Speed*” is then displayed
+-	Disinfect the inner surfaces of the BSC with Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates
+-	Allow the cabinet to run for at least 15 minutes with no activity
+-	Switch on the light by pressing on  <img src="img/img14.png" width="30"> 
+-	Work as clean as possible, please read the [Good practices in a BSC](#good-practices-in-a-bsc) before starting your experiment
+-	When work is done, disinfect the inner surfaces of the BSC Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates
+-	Switch off the light by pressing on <img src="img/img14.png" width="30"> 
+-	Allow the cabinet to run for at least 15 minutes with no activity to purge air contaminants from the cabinet
+-	Stop the ventilation by pressing on <img src="img/img13.png" width="30">   
+-	Close the sash by pressing on <img src="img/img15.png" width="30"> 
+
+**UV decontamination**
+
+We do not recommend using UV-light because it can give a false sense of security and you should be aware of the limitations of the UV-light. The UV-light act only on surfaces exposed to the UV light, but all the surfaces which are in the shadow are not disinfected. UV decontamination can be done in addition to chemical disinfection when work is completed.
+-	Make sure the UV lamp is plugged in the socket localized inside the cabinet, on the left side
+<div align="center">
+<img src="img/img16.png" width="150">
+</div>
+
+-	In the case where UV lights are used, close the sash by pressing on <img src="img/img15.png" width="30"> 
+-	Press on   to start the UV cycle <img src="img/img17.png" width="30"> 
+-	The screen displays “*UV OFF / + = Button / - = Timer*”, press on <img src="img/img18.png" width="30"> 
+-	The display then shows “*Set time / 20 min*”
+-	Click on <img src="img/img19.png" width="30">, the screen starts a count down from “*UV light in 10 seconds*”
+-	The UV light will then stay on for 20 minutes
+-	Turn the key tp switch the power off 
+
+## How to use LAMSYSTEMS BSC
+<div align="center">
+<img src="img/img20.png" width="200">
+</div>
+
+Control panel:
+<div align="center">
+<img src="img/img21.png" width="300">
+</div>
+
+**Turning on/off the cabinet**
+-	Open the sash in working position using the arrow on the control panel
+-	Start the ventilation by pressing on “FAN ON”
+-	Disinfect the inner surfaces of the BSC with Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates 
+-	Allow the cabinet to run for at least 15 minutes with no activity
+-	Turn the illumination on
+-	Work as clean as possible, please read the [Good practices in a BSC](#good-practices-in-a-bsc) before starting your experiment
+-	When work is done, disinfect the inner surfaces of the BSC Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates
+-	Turn the illumination off
+-	Allow the cabinet to run for at least 15 minutes with no activity to purge air contaminants from the cabinet
+-	Stop the ventilation by pressing on “FAN OFF” 
+-	Close the sash using the arrow
+
+**UV decontamination**
+
+We do not recommend using UV-light because it can give a false sense of security and you should be aware of the limitations of the UV-light. The UV-light act only on surfaces exposed to the UV light, but all the surfaces which are in the shadow are not disinfected. UV decontamination can be done in addition to chemical disinfection when work is completed.
+<div align="center">
+<img src="img/img22.png" width="400">
+</div>
+
+1.	Open the front sash and detach the armrest (if there)
+2.	Pull the UV unit towards you
+3.	Open the UV unit
+4.	Store the armrest in the dedicated place
+6.	Push the open UV unit
+7.	Place the UV unit inside the cabinet chamber, close the front sash and set the UV for about 30 minutes by pressing on “UV unit on/off”
+
+## How to use BIOAIR BSC
+<div align="center">
+<img src="img/img23.png" width="200">
+</div>
+
+The BSC may operate in 2 modes:
+-	***MODE 1*** – SAFE OPERATING: Default operating condition
+-	***MODE 2*** – STAND BY (UNSAFE): Language selection, date and time setting, switch on UV light cycle
+
+Control panel:
+<div align="center">
+<img src="img/img24.png" width="400">
+</div>
+<div align="center">
+<img src="img/img25.png" width="350">
+</div>
+
+**Turning on/off the cabinet**
+
+***MODE 1*** – SAFE OPERATING
+-	Turn the cabinet on by the key switch. If the self-test is passed, the screen is shown to decide within 10 s whether to continue accessing in MODE 1 (default access without action) or switch to MODE 2 
+<div align="center">
+<img src="img/img26.png" width="180">
+</div>
+
+-	Wait for WARM UP TIME
+-	Ventilation started with an audible and visual alarm.
+-	The front window is lifted automatically reaching the working position
+-	If the WARM UP has been successful, the SAFE OPERATING screen will appear
+<div align="center">
+<img src="img/img27.png" width="180">
+</div>
+
+-	The alarm will stop when the correct flow value is reached
+-	The following are enabled: up and down front window movement, light, service socket, gas solenoid valve (if installed) and ECO mode
+-	Disinfect the inner surfaces of the BSC with Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates 
+-	Allow the cabinet to run for at least 15 minutes with no activity
+-	Turn the light on
+-	Work as clean as possible, please read the [Good practices in a BSC](#good-practices-in-a-bsc) before starting your experiment
+-	When work is done, disinfect the inner surfaces of the BSC Bacillol 30 foam or tissues. Do not use bleach: it might corrode the stainless-steel plates
+-	Allow the cabinet to run for at least 5 minutes with no activity to purge air contaminants from the cabinet
+-	Close the sash using the arrows and turn off the light
+-	Turn the ventilation off via the key switch
+
+**UV decontamination**
+
+We do not recommend using UV-light because it can give a false sense of security and you should be aware of the limitations of the UV-light. The UV-light act only on surfaces exposed to the UV light, but all the surfaces which are in the shadow are not disinfected. UV decontamination can be done in addition to chemical disinfection when work is completed.
+
+The UV decontamination is performed under ***MODE 2***
+-	Turn the cabinet on by the key switch. After the self-test, press MODE within 10 seconds in order to switch to MODE 2 
+-	Wait for STAND-BY message
+<div align="center">
+<img src="img/img28.png" width="180">
+</div>
+
+-	Close the front sash
+-	Check fluorescent light be off
+-	Press UV key
+-	Select the UV cycle time by pressing <img src="img/img29.png" width="20">  key (UV).
+-	Default setting is 10 minutes: press again <img src="img/img29.png" width="20">   key (UV) to increase the cycle time until 30 minutes
+-	Press <img src="img/img30.png" width="35">  key (SOCKET) to confirm. Press again the SOCKET key to start the UV cycle immediately or press <img src="img/img29.png" width="20">   key (UV) to program the starting time.
+-	Display will show the remaining time of the UV cycle
+-	When the UV cycle is completed, the UV lamp turns off and the display will show “UV decontam. completed”
+-	Press  <img src="img/img31.png" width="15"> key (LIGHT) to exit and return to MODE 2 operating message
+-	Turn the cabinet off via the key switch
+
+## Maintenance of BSC
+
+**Before and after each use**, the BSC must be disinfected by the user with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should use Bacillol 30 foam or tissues. Corrosive chemicals such as bleach should be avoided (bleach corrodes stainless steel), but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels.
+
+**Once a month**, each BSC should be deeply cleaned with an appropriate disinfectant such as Bacillol 30 and 70% ethanol combined. During this deep cleaning, remove the removable inner surfaces, disinfect the surface under the plates, the inside walls, the grills and the collecting pan. Disinfect and put back the removable inner surfaces. Don’t use corrosive chemicals on stainless-steel plates. Fill the form beside the BSC with the date, your initials and the maintenance performed.
+
+**Once a year**, an operational qualification is realised on each BSC. This qualification consist of :
+- a check of the efficiency of the airflow to ensure that the inflow and the downflow velocities that are displayed by the cabinet are correctly measured.
+- a check of the integrity of the HEPA filters.
+- a measure of the cleanliness of the air via a particle counting
+
+After a successful qualification, a sticker is placed on the BSC with the due requalification date. Before using a BSC, please verify that the BSC has been qualified during the past 12 months.
+
+## How to report an issue with a BSC
+
+If you notice an issue with a BSC, please send [a ticket](https://service.uni.lu/sp?id=sc_cat_item&sys_id=be0873f8db7070505c72ef3c0b96199f&sysparm_category=af924f17dbac38105c72ef3c0b96194b) to the Instrument Care Team. Within this ticket, include a description of the issue, the LCSB number of the BSC, the location, the type of the instrument (protective enclosures) and specify if there is a warning signal. In such case, place a sign “*Don’t use the instrument*” on the BSC and wait for the Instrument Care Team reply or intervention before using it again.
\ No newline at end of file