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Commit 70672d03 authored by Annegrät Daujeumont's avatar Annegrät Daujeumont
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Update external/lab-equipment/utilization-of-bsc/utilization-of-bsc.md

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......@@ -22,8 +22,8 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
- The user must wear **lab coat** and **gloves** while using a BSC.
< img src="img/image1.jpeg" width="40" >
< img src="img/image2-jpeg" width="40" >
<img src="img/image1.jpeg" width="40">
<img src="img/image2-jpeg" width="40">
- The BSC should be **empty** when you start working. If material is stored inside the BSC (for instance micropipettes), they must be disinfected when you switch on the BSC.
......@@ -31,12 +31,12 @@ If you have any question regarding the usage of a BSC, please send a ticket to t
- The BSC must be **decontaminated before and after each use**. The interior surfaces should be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. 70% ethanol is not effective against yeast and fungi, you should **use Bacillol 30 foam or tissues**.
< img src="img/image3.jpeg" >
< img src="img/image4.jpeg" >
<img src="img/image3.jpeg">
<img src="img/image4.jpeg">
Corrosive chemicals such as bleach should be avoided, but if used, should be followed with a wipe down of sterile water or 70% ethanol. Wipe the work surface, the inside walls, below the work surface and the collecting pan. Do not use non-sterile paper towels.
< img src="img/image5.png" >
<img src="img/image5.png">
- Allow the cabinet to run for at least **10 minutes** after switching on.
......@@ -44,13 +44,13 @@ Corrosive chemicals such as bleach should be avoided, but if used, should be fol
- Put **Incidin PLU**S in **the aspiration pump** and disinfect the pump tubing with **Ethanol 70% or Bacillol 30**
< img src="img/image6.jpeg" >
<img src="img/image6.jpeg">
- Control the cells in the **microscope** before starting to work to **detect contamination** in your cell culture (change in the medium color, cloudiness of the medium cross-contamination with other cell lines, ...).
- Set up workspace in a direction **from clean to dirty**.
< img src="img/image7.png" >
<img src="img/image7.png">
- **Never cover the front or rear grills** with any material, even temporarily.
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