To protect every person present in the laboratories from any contamination or exposure to hazardous materials, chemical or biological, each person entering the laboratories has to wear the apprioriate Personnal Protective Equipment (PPE).
Every LCSB employees, students and visitors are responsible
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- Appropriate lab coat
- Appropriate disposable gloves (when handling biological materials or chemicals)
Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**
It is not that trivial to safely wear and remove disposable gloves without becoming contaminated, please watch the video in [DOCEBO platform](https://unisupport.docebosaas.com/learn/course/52/play/332:103/how-to-wear-and-remove-gloves;lp=3).
Not sure which glove to use? **Consult the chemical's Safety Data Sheet (SDS) or the [safety officer](https://service.uni.lu/sp?id=sc_cat_item&sys_id=f140d745dbe83c905c72ef3c0b96194e&sysparm_category=cca7f2c1db683c905c72ef3c0b961940) for guidance.**
It is not that trivial to safely wear and remove disposable gloves without becoming contaminated, please watch the video in [DOCEBO platform](https://unisupport.docebosaas.com/learn/course/52/play/332:103/how-to-wear-and-remove-gloves;lp=3 ).
- Specific PPEs depending on the performed activity (faceshield, thermal gloves, themal appron,...)
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## PPE Requirements
### Classical laboratories activities
### 1. Classical laboratories activities
For regular biomolecular, chemical, cell culture or microbiology work in BSL 1 or BSL 2 laboratories, the mandatory PPEs are
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### Work with stronger chemicals
### 2. Work with stronger chemicals
When handling stronger chemicals, such as work with DNA labelling reagents.
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### Work with strong chemical risk
### 3. Work with strong chemical risk
Dealing with strong chemicals such as strong acids, bases,... requires specific gloves providing specific protection, on top of the regular PPEs.
Those gloves are not disposable gloves.
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### Autoclave unloading and other hot thermal risks
### 4. Autoclave unloading and other hot thermal risks
On top of the mandatory PPEs for BSL1 work, wear the **Ansell Gladiator 16-650 gloves** located next to the autoclaves to protect you against hot thermal hazard. Those gloves are not disposable gloves.
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### Automated cryostorage
### 5. Automated cryostorage
On top of the mandatory PPEs for BSL1 work, it is mandatory to wear:
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### Distribution of Liquid Nitrogen in a Dewar
### 6. Distribution of Liquid Nitrogen in a Dewar
On top of the mandatory PPEs for BSL1 work, it is mandatory to wear:
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### Usage of Liquid Nitrogen in open Dewars
### 7. Usage of Liquid Nitrogen in open Dewars
Some experiments require to snap freeze the samples in Liquid Nitrogen or to keep samples frozen in Liquid Nitrogen during the manipulation. Having Liquid Nitrogen in an open Dewar on the bench top requires to wear on top of the regular PPEs:
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### Other cold thermal risks
### 8. Other cold thermal risks
When handling samples in the -80°C freezers, in the -150°C freezer, in the cryopod or in dry-ice, it is recommended to wear, on top of the regular mandatory PPEs cotton white gloves to be worn under the regular nitrile glove. It will enable an improved dexterity compared to the regular cryo-gloves. Those cotton gloves are not disposable and should be kept in your lab coat.
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### Spill management
### 9. Spill management
Spill management requires extra protection that you can find in the spill kit. On top of the regular PPEs, you have to wear:
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### Work with non tested biological samples
### 10. Work with non tested biological samples
Collective protective equipment is always the preferred option to be protected against airborne biological hazards. Therefore, biological hazards should be manipulated under biosafety cabinets. However, if after discussion with the safety officer and/or after a risk assessment made in collaboration with the safety officer lead to the impossibility to work under a biosafety cabinet, it is mandatory to wear, on top of the regular PPEs:
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### Work with non-toxic smelling samples
### 11. Work with non-toxic smelling samples
To avoid unpleasant smells in the laboratories, it is recommended to work with non-toxic samples under a chemical hood or an aspiration arm. However, if after discussion with the safety officer and/or after a risk assessment made in collaboration with the safety officer lead to the impossibility to work under a chemical hood or an aspiration arm, it is recommended to wear, on top of the regular PPEs:
