Skip to content
Snippets Groups Projects
Commit 941b430d authored by Valentina Galata's avatar Valentina Galata
Browse files

rm CHANGELOG and not used config

parent 10e83e92
No related branches found
No related tags found
1 merge request!76Merge "cleanup" branch with "master" branch
- All files uploaded to new repository "ONT_pilot_gitlab" for the ONT analyses
- Snakefile updated with correct paths for short-read mapping against IGC and HG38 reference databases
\ No newline at end of file
steps: "assembly_annotation metaT"
# steps: "assembly_annotation mmseq metaT mapping binning taxonomy"
data_dir: "data"
results_dir: "results"
db_dir: "dbs"
runs:
first: "S1_SizeSelected"
second: "S3_Gtube"
# third: "20181108_0827_test"
barcodes: ["barcode06", "barcode07", "barcode08", "barcode09", "barcode10"]
assemblers: ["flye"]
p7zip:
bin: "/home/users/claczny/apps/software/p7zip_16.02/bin/7za"
threads: 4
ont_fast5_api:
single_to_multi_fast5:
bin: "single_to_multi_fast5"
batch: 8000
threads: 8
flowcell: "FLO-MIN106"
kit: "SQK-LSK109"
#barcodes: ["barcode06", "barcode07", "barcode08", "barcode09", "barcode10"]
barcodes: ["barcode07"]
guppy_cpu:
path: "/scratch/users/claczny/ont/apps/software/ont-guppy-cpu-3.1.5_linux64/bin"
bin: "/scratch/users/claczny/ont/apps/software/ont-guppy-cpu-3.1.5_linux64/bin/guppy_basecaller"
version: "cpu-3.1.5"
config: "dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg"
cpu_threads: 28
guppy_gpu:
path: "/home/users/sbusi/apps/ont-guppy/bin"
bin: "set +u; source ~/.bashrc; set -u; ml compiler/LLVM system/CUDA && /home/users/sbusi/apps/ont-guppy/bin/guppy_basecaller"
version: "3.6.0+98ff765"
config: "dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg"
hac_config: "dna_r9.4.1_450bps_hac.cfg"
records_per_fastq: 8000
chunk_size: 1000
chunks_per_runner: 1000
num_callers: 4
runners_per_device: 2
gpu_device: "cuda:0"
cpu_threads: 28
guppy_barcoder:
path: "/home/users/sbusi/apps/ont-guppy/bin"
bin: "set +u; source ~/.bashrc; set -u; ml compiler/LLVM system/CUDA && /home/users/sbusi/apps/ont-guppy/bin/guppy_barcoder"
version: "3.4.5+fb1fbfb"
records_per_fastq: 8000
threads: 8
nanostats:
short_reads_prefix: "/scratch/users/sbusi/ONT/cedric_ont_basecalling/2019_GDB/data/raw/short_reads"
#short_reads_prefix: "/mnt/isilon/projects/lcsb_sequencing/transfer/bioecosystem/Rashi/2019/Apr/fastq"
metaT_prefix: "/scratch/users/sbusi/ONT/cedric_ont_basecalling/2019_GDB/data/metaT"
fastp:
min_length: 40
minimap2:
threads: 16
igc:
uri: "parrot.genomics.cn/gigadb/pub/10.5524/100001_101000/100064/1.GeneCatalogs/IGC.fa.gz"
hg38:
uri: "ftp.ncbi.nlm.nih.gov/genomes/all/GCF/000/001/405/GCF_000001405.38_GRCh38.p12/GCF_000001405.38_GRCh38.p12_genomic.fna.gz"
genomecov:
bin: "bedtools genomecov"
compute_avg_coverage:
bin: "scripts/coverage.awk"
bwa:
threads: 24
long_reads_index:
opts: "-aY -A 5 -B 11 -O 2,1 -E 4,3 -k 8 -W 16 -w 40 -r 1 -D 0 -y 20 -L 30,30 -T 2.5"
samtools:
sort:
threads: 4
chunk_size: "4G"
view:
threads: 4
flye:
bin: "flye"
threads: 27
genome_size: "1g"
operams:
bin: "set +u; source ~/.bashrc; set -u; ml lang/Perl lang/R && perl /scratch/users/claczny/ont/apps/software/OPERA-MS/OPERA-MS.pl"
threads: 28
megahit:
threads: 28
nonpareil:
memory: 4096
threads: 14
medaka:
threads: 28
racon:
threads: 28
rebaler:
threads: 28
diamond:
threads: 28
db: "/mnt/isilon/projects/ecosystem_biology/NOMIS/DIAMOND/new_nr.dmnd"
metaspades:
threads: 28
mmseq2:
threads: 24
# Define sample names
#samples: ["flye", "megahit", "metaspades_hybrid"]
# samples: ["flye", "megahit"]
# samples: ["metaspades_hybrid"]
binning_samples: ["flye", "megahit", "bwa_sr_metaspades_hybrid", "bwa_lr_metaspades_hybrid", "bwa_merged_metaspades_hybrid", "mmi_sr_metaspades_hybrid", "mmi_lr_metaspades_hybrid", "mmi_merged_metaspades_hybrid"]
# Hybrid assembler
hybrid_assembler: "metaspades_hybrid"
# Directory where fastq files are
#data_dir: "/scratch/users/sbusi/ONT/cedric_ont_basecalling/Binning"
# Directory to save the output to
#results_dir: "/scratch/users/sbusi/ONT/cedric_ont_basecalling/Binning"
# Number of cpus or threads to use
threads: 28
# Path to the the 140GB Kraken2 database
kraken2_database: "/scratch/users/bkunath/Kraken2/maxikraken2_1903_140GB/"
# Path to DAS_Tool
DAS_Tool:
path: "/home/users/sbusi/apps/DAS_Tool-master"
bin: "/home/users/sbusi/apps/DAS_Tool-master/src/"
# Path to DAS_Tool database
dastool_database: "/home/users/sbusi/apps/DAS_Tool-master/db/"
# Mapping options
bwa:
threads: 24
long_reads_index:
opts: "-aY -A 5 -B 11 -O 2,1 -E 4,3 -k 8 -W 16 -w 40 -r 1 -D 0 -y 20 -L 30,30 -T 2.5"
samtools:
sort:
threads: 4
chunk_size: "4G"
view:
threads: 4
minimap2:
threads: 24
# Path to GTDBTK database
GTDBTK:
DATA: "/home/users/sbusi/apps/db/gtdbtk/release89"
# Rscript path
Rscript: "/home/users/sbusi/apps/miniconda3/envs/dastool/bin/"
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment